iBiology Videos

To understand the relationship between RNA localization and translation, it is necessary to visualize the movement of the RNA in real time. In his second lecture, Singer explains how his lab found an ingenious way to label RNAs in live cells and follow their movement. In the nucleus, RNA moves by diffusion and stochastically finds a nuclear pore to exit. In the cytoplasm, however, it is a different story. RNA, in a translationally repressed state, undergoes directed movement along the cytoskeleton. Once it reaches its destination, translation is activated. Singer’s lab showed that neuronal stimulation leads to ß-actin mRNA localization to dendritic spines, followed by translation, and stabilization of the synapse; events that are crucial for memory and learning.

In this series of lectures, Dr. Robert Singer explains how it is possible to follow a single mRNA molecule from its birth to its death. Singer begins by explaining that cell cultures are heterogeneous and analyzing single cells provides spatial and temporal information not available from bulk analysis. He outlines how his lab developed techniques such as fluorescence in situ hybridization (FISH) that have allowed them to measure gene expression in single cells. Using these techniques, they discovered that some RNAs localize to specific areas in the cell and RNA localization is linked to its regulation and function. For example, ß-actin mRNA localization to the leading edge of fibroblasts aids in cell motility. Singer’s lab identified “zip code” regions in mRNAs that are responsible for regulating mRNA localization.

In his second talk he reviews makeup of the plant cell wall and how hemicellulose, cellulose, and lignin can be broken down for use in aviation biofuel production. He describes the Joint BioEnergy Institute (JBEI) approach to maximize carbon efficiency at every step in the production of biofuels, from developing and feedstocks, deconstructing biomass, and use of microbes and enzymes to generate target intermediates for fuels. A major part of optimizing this process is the development of a one-pot process, in which conversion of biomass to fuel can be achieved in a single chamber.

Biofuels have been around for decades, but interest in them has grown considerably in recent years due to climate change. While they have the potential to provide a sustainable and renewable way to power our transportation systems, Dr. Blake Simmons points out that in order to become a viable replacement for fossil fuels, biofuels must also be efficient and affordable at global scales. In his first talk, he provides an overview of the advantages of using biofuels and walks through the biochemical processes required in order to convert biomass into usable fuels. He also discusses the ideal properties for advanced biofuels, the projected markets for these fuels in the coming years, and some of the key challenges associated with optimization of advanced biofuel production.

There are many career options open to PhD scientists, however it can be difficult to determine which career is right for you. In a series of talks, Dr. Alexandra Schnoes describes how internships for graduate students and postdocs might be a good way to explore careers and help make an informed career decision. She discusses the benefits of internships and how a graduate student or postdoc might go on one. She covers the basics of finding and applying for internships. She also talks about the process of talking to your advisor about going on an internship, and how to get the most out of your internship experience when you are on it.

There are many career options open to PhD scientists, however it can be difficult to determine which career is right for you. In a series of talks, Dr. Alexandra Schnoes describes how internships for graduate students and postdocs might be a good way to explore careers and help make an informed career decision. She discusses the benefits of internships and how a graduate student or postdoc might go on one. She covers the basics of finding and applying for internships. She also talks about the process of talking to your advisor about going on an internship, and how to get the most out of your internship experience when you are on it.

In his Part 3, Rutter emphasizes the challenge of mitochondrial protein synthesis. How do the components of the electron transport chain (ETC) assemble in the right stoichiometry at the right time? Rutter introduces the LYR family of proteins, which aid assembly of ETC components. LYR proteins interact with a common binding partner, the acyl carrier protein (ACP), via a unique fatty acyl moiety on ACP. Rutter’s group showed that ACP acylation is necessary for assembly of the ETC and activation of oxidative phosphorylation.

In Part 2 of his talk, Rutter describes his group’s work to unravel the relationship between the activity of the Mitochondrial Pyruvate Carrier (MPC) and the behavior of numerous cell types, including cancer and stem cells. His group found that forced expression of the MPC in multiple stem cell models led to reduced “stemness” and proliferative capacity, and that MPC inhibition could promote organoid formation in culture and tumor formation in vivo. These data indicate an important link between mitochondria, metabolism, and cell behavior.

Mitochondria are integral to the metabolism of eukaryotic cells, yet many of their properties are not fully understood. In Part 1 of this iBioSeminar, Dr. Jared Rutter lays out the foundational knowledge of mitochondrial structure and origin, and shares what is currently known about mitochondrial roles in metabolism, protein homeostasis, and signaling. He ends by highlighting a focus of his research group: to unravel the functions of uncharacterized mitochondrial proteins.

Part 2: Rooney-Varga discusses the need for integrating climate change into biology education and why it can be difficult to understand and teach. She explains that introducing simulation-based role-playing games to students is an effective and fun way to teach how decisions and actions taken today will impact climate change in the future.