The podcast discussion comprehensively explores the Application Layer (OSI Layer-7), focusing on incident response strategies. It examines various protocols, including HTTP, HTTPS, DNS, SMTP, and FTP, alongside application-specific APIs. It details incident response tactics for common threats like web application attacks, injection flaws, cross-site scripting, CSRF, API abuse, and zero-day exploits, using real-world case studies such as the Equifax breach and Log4Shell. Furthermore, it outlines the use of playbooks and runbooks for operational responses, aligning with industry standards like NIST, SANS, and ISO, and addresses forensics at Layer-7 through log analysis and packet capture. The discussion also projects future threats, including AI-driven attacks and cloud-native application security challenges, emphasising the importance of organisational readiness.