Listen In - Bitesize Bio Webinar Audios

Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen.

In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper.

These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers.

Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art.

Tune in to discover how you can turn your passion for science and art into a fulfilling career.

Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In.

Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.

Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels.

Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training.

Watch the full presentation here: https://microscopyfocus.com/five-color-sted/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice.

Learn how to tackle issues like:

• Career disruption
• Reason for choosing your place of work
• Track record relative to opportunity
• Consumer involvement

This episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant!

Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores.

But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.

Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags.

These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.

Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control.

In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset.

Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively.

Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions.

Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.

Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.

Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.

Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

Single cell sequencing samples must be prepared to the highest possible standard.

Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing.

In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample.

You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.

Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In.

She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements.

As well as learning how to work out your h-index, you will learn how to describe the impact of your papers.

Watch the full presentation here: https://events.bitesizebio.com/how-to-effectively-communicate-your-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment. 

Because of their diverse physiological and pathophysiological roles, GPCRs are also major drug targets.

Our understanding of GPCR signaling has evolved from single receptors at the cell surface activating specific heterotrimeric G protein pathways, to an increasingly complex receptor signaling system.  However, the complexity of GPCR signaling is translated to specific cellular and physiological responses is still unclear.

In this episode, we explore some of the latest endeavors to uncover GPCR signaling mechanisms through a wide range of imaging applications, from analyzing single molecules to the identification of new subcellular signal platforms.

Watch the full presentation here: https://microscopyfocus.com/imaging-gpcr-activity-unravelling-mechanisms-from-molecules-to-morphology/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

As a researcher, one of the most crucial skills to develop is the ability to perform an efficient yet thorough review of manuscripts.

Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and handle tricky issues such as image and data manipulation.

In this episode of Listen In, Dr. Johanna Ahlskog discusses the essential skills all reviewers and researchers need, emphasizes the critical importance of the review process, and offers general considerations when reviewing articles.

Watch the full presentation here: https://events.bitesizebio.com/critical-analysis-of-research-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/