The agar to bag transfer method involves cultivating mycelium on a nutrient-rich agar medium within a sterile petri dish. Once the mycelium has established healthy growth, a small portion is carefully transferred to a sterilized bag filled with a substrate like grain or sawdust. The mycelium then colonizes the substrate, eventually producing a flush of mushrooms. This method is prized for minimizing contamination risks and maximizing yield potential.

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