When English settlers finally returned to Roanoke, they expected to reunite with the colony they had left behind. Instead, every man, woman, and child had disappeared. There were no signs of violence, no graves, and almost no clues—only one mysterious word carved into a wooden post. In this episode, we revisit America's oldest missing persons case, examining the evidence, archaeological discoveries, and competing theories that continue to fuel one of history's greatest unsolved disappearances.