It's Sheriff Wario vs Ran the Witch (played by Teens Choice Award-nominated Asian Superstar Scarlett Johansson).

In our first episode of the Backlog, we revisit 2 of our childhood multiplayer favorites that we were forced to play alone. Flocky introduces Pocky to the chaos that is playing Mario Party with friends (it's just like watching your favorite VTuber do it!), while Pocky introduces us to one of his childhood favorites, the competitive shoot-em-up, Twinkle Star Sprites. It's the same nonsense you love, just with video games now!