Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions.

Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.

Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.

Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.

Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com