It just means, "no." It's a negative response to a question or offer. 

"Are you available Tuesday evening?" 
"No."

"Open to a booty call?"
"No."

"Do you like radishes?"
"No."

I'm not free Tuesday night because I have a dinner for friends planned. 

I don't particularly like booty call relationships—yes, I have tried them in the past. 

And radishes are just disgusting. 

Find the full transcript for this episode here. https://datingkinky.com/blog/communication/what-does-no-mean/