Ask Stago (english version)

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. In today episode, our expert Vicki, will give you all her tips to collect and prepare you sample in the best way.  From sample, material or storage be sure to provide valid sample to your lab.  A podcast to share with your colleague, nurses or any person in charge of the preparation of the sample in your facility.  

As usual, don’t forget to send any question you may have to [email protected], we will be glad to answer to it.  

To learn more: Continue with our podcast dedicated to the HIL pre-analytical check https://www.podcastics.com/podcast/episode/9-how-to-manage-hil-samples-in-the-coagulation-laboratory-37748/ 

Literature: 

• Stability of coagulation proteins in frozen plasma. B. Woodhams, O. Girardot, MJ Blanco and Y.Gourmelin, Blood coagulation and fibrinolysis 2001, 12:229-236 
• Effects of storage time and temperature on coagulation tests and factors in fresh plasma Limin Feng, Ying Zhao, Hongcan Zhao & Zhexin Shao Scientific Reports 4, Article number: 3868 (2014) doi:10.1038/srep03868 
• Quality Standards for Sample Collection in Coagulation Testing Giuseppe Lippi, M.D. 1 Gian Luca Salvagno, M.D. 2 Martina Montagnana, M.D. 2 Gabriel Lima-Oliveira, M.D. 2 Gian Cesare Guidi, M.D. 2 Emmanuel J. Favaloro, Ph.D., M.A.I.M.S., F.F.Sc. (RCPA) 3 Semin Thromb Hemost 2012;38:565–575. 
• Quality in Laboratory Hemostasis and Thrombosis. S.Kitchen, JD Olson, E.Preston. Wiley-Blackwell 2009 
• Quality Standards for Sample Processing,Transportation, and Storage inHemostasis Testing Dorothy M. Adcock Funk M.D., Giuseppe Lippi M.D., Emmanuel J. Favaloro Ph.D., Semin Thromb Hemost 2012;38:576–585. 
• Pre-analytical Variables in Coagulation Testing Associated With Diagnostic Errors in Hemostasis Emmanuel J. Favaloro, PhD, MAIMS, FFSc (RCPA); Dorothy M. (Adcock) Funk, MD; Giuseppe Lippi, MD DisclosuresLab Med. 2012;43(2):1-10.  
• Effects of haemolysis, icterus and lipaemia on coagulation tests as performed on Stago STA-Compact-Max analyser, A. WOOLLEY, J.-L. GOLMARD, S. KITCHEN, 2016 John Wiley & Sons Ltd, Int. Jnl. Lab. Hem. 
• Recommandation préanalytiques en hémostase; GFHT 2017-2018 
• CLSI Collection, Transport and Processing of Blood Specimens for Testing Plasma-based Coagulation Assays and Molecular Hemostasis Assays; Approved Guideline – Fifth Edition. CLSI Document H21-A5; Wayne, PA: Clinical and Laboratory Standards Institute; 2008 
• Alesci S, Borggrefe M, Dempfle CE. Effect of freezing method and storage at -20⁰C and -70⁰C on prothrombin time, aPTT and plasma fibrinogen levels. Thromb Res. 2009;124(1):121-126. 
• van den Besselaar AM, Witteveen E, van der Meer FJ. Long-term stability of frozen pooled plasmas stored at -70°C, -40°C, and -20°C for prothrombin time and International Normalized Ratio (INR) assessment. Thromb Res. 2013 Apr;131(4):349-51. doi: 10.1016/j.thromres.2013.01.020. Epub 2013 Feb 12. PMID: 23411127. 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. In today episode, our expert Alex, will discuss with us how to determine factor levels in hemophilia patients.  

Literature sources: 

• Alfonso Iorio,Jeffrey S. Stonebraker, Hervé Chambost, et al.  Establishing the Prevalence and Prevalence at Birth of Hemophilia in Males: A Meta-analytic Approach Using National Registries. Ann Intern Med.2019;171:540-546. [Epub ahead of print 10 September 2019]. doi:10.7326/M19-1208 
• Blanchette, VS, Key, NS, Ljung, LR, Manco‐Johnson, MJ, van Den Berg, HM, Srivastava, A, for the Subcommittee on Factor VIII, Factor IX and Rare Coagulation Disorders. Definitions in hemophilia: communication from the SSC of the ISTH. J Thromb Haemost 2014; 12: 1935– 9. 
• Baker, P., Platton, S., Gibson, C., Gray, E., Jennings, I., Murphy, P., Laffan, M. and (2020), Guidelines on the laboratory aspects of assays used in haemostasis and thrombosis. Br. J. Haematol., 191: 347-362. https://doi.org/10.1111/bjh.16776 
• CLSI, Determination of coagulation factor activities using the one-stage clotting assays. 2nd edition. CLSI guideline H48. Wayne PA: Clinical and Laboratory Standards Institute, 2016. 
• College of American Pathologists. Hematology and Coagulation Checklist. Northfield, IL: College of American Pathologists; 2018 
• Riley PW, Gallea B, Valcour A. Development and implementation of a coagulation factor testing method utilizing autoverification in a high‑volume clinical reference laboratory environment. J Pathol Inform 2017;8 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. In today episode, our expert Tara Simpson, will cover with us how to establish and control an assay reference range.  

Literature sources: 

• Henny J. Établissement et validation des intervalles de référence au laboratoire de biologie médicale. Ann Biol Clin 2011 ; 69(2) : 229-37 doi:10.1684/abc.2011.0537 
• Gardiner, C, Coleman, R, de Maat, MPM, et al. International Council for Standardization in Haematology (ICSH) laboratory guidance for the evaluation of haemostasis analyser‐reagent test systems. Part 1: Instrument‐specific issues and commonly used coagulation screening tests. Int J Lab Hematol. 2020; 00: 1– 15. https://doi.org/10.1111/ijlh.13411
• CLSI. EP28-A3C Defining, Establishing and Verifying Reference Intervals in the Clinical Laboratory: Approved Guideline. 3rd ed. Wayne, PA: CLSI; 2008;28:1‐76 
• Castellone DD. Establishing reference intervals in the coagulation laboratory. Int J Lab Hematol. 2017;39(Suppl 1):121-127 
• CLSI. Measurement procedure comparison and Bias Estimation Using Patient samples. 3rd ed. CLSI guideline EP09c. Wayne PA: Clinical and Laboratory Standards Institute; 2018 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. 
In today episode, our expert Draga, will give you all her tips to have the perfect calibration.  

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, the weekly podcast about hemostasis.   

This week, Cécile Hourquet and Audrey Carlo will discuss about viscoelastic testing and the usage of it with our expert Wim Houdijk.   

Literature: 

• Hartert H. Blutgerinnungsstudien mit der Thrombelastographie, einem neuen Untersuchungsverfahren.Klin Wochenschr. 1948;26:577-583.  
• Sayce AC, Neal MD, Leeper CM. Viscoelastic monitoring in trauma resuscitation Transfusion. 2020;60 (Suppl 6):S33-S51.  
• Agarwal S, Abdelmotieleb M. Viscoelastic testing in cardiac surgery. Transfusion. 2020;60 (Suppl 6):S52-S60.  
• Tetsuro Sakai.Viscoelastic testing in liver transplantation. Transfusion. 2020;60 (Suppl 6):S61-S69.  
• Waters JH. The role of viscoelastic testing in the management of the parturient. Transfusion. 2020;60 (Suppl 6):S70-S74.  
• Carll T, Wool GD. Basic principles of viscoelastic testing. Transfusion. 2020;60 (Suppl 6):S1-S9.  
• Cohen T, Haas T, Cushing MM. The strengths and weaknesses of viscoelastic testing compared to traditional coagulation testing. Transfusion. 2020;60 (Suppl 6):S21-S28.  
• Meco M, Montisci A, Giustiniano E, Greco M, Pappalardo F, Mammana L, Panisi P, Roscitano C, Cirri S, Donatelli F, Albano G. Viscoelastic blood tests use in adult cardiac surgery: meta-analysis, meta-regression, and trial sequential analysis. J Cardiothorac Vasc Anesth. 2020;34:119-127.  
• Allen TW, Winegar D, Viola F. The Quantra system and SEER sonorheometry. In Moore HB, et al. (eds.), Trauma induced coagulopathy. Springer Nature Switzerland AG 2021, pages 693-704. 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, the weekly podcast for Hemostasis laboratory professionals.   

This week, with our expert Paul Riley, Scientific Business Development Manager, Cécile Hourquet and Audrey Carlo will cover the usage of fibrin related markers in the diagnosis of disseminated intravascular coagulopathy.   

Litterature: 

• Baglin T. Disseminated intravascular coagulation: diagnosis and treatment. BMJ 1996; 312: 683-7 
• Iba T, Di Nisio M, Thachil J, Wada H, Asakura H, Sato K, Kitamura N, Saitoh D. Revision of the Japanese Association for Acute Medicine (JAAM) disseminated intravascular coagulation (DIC) diagnostic criteria using antithrombin activity. Crit Care. 2016 Sep 14;20:287 
• Taylor FB Jr, Toh CH, Hoots WK, Wada H, Levi M; Scientific Subcommittee on Disseminated Intravascular Coagulation (DIC) of the International Society on Thrombosis and Haemostasis (ISTH). Towards definition, clinical and laboratory criteria, and a scoring system for disseminated intravascular coagulation. Thromb Haemost. 2001 Nov;86(5):1327-30 
• JAAM; Iba T, Di Nisio M, Thachil J, Wada H, Asakura H, Sato K, Kitamura N, Saitoh D. Revision of the Japanese Association for Acute Medicine (JAAM) disseminated intravascular coagulation (DIC) diagnostic criteria using antithrombin activity. Crit Care. 2016 
• Sep 14;20:287 
• Boral BM, Williams DJ, Boral LI. Disseminated Intravascular Coagulation. Am J Clin Pathol 2016; 146: 670-80 
• Wada H, Sakuragawa N. Are fibrin-related markers useful for the diagnosis of thrombosis? Semin Thromb Hemost 2008; 34: 33-8 
• Toh JMH, Ken-Drorb G, Downey D, Abram ST. The clinical utility of fibrin-related biomarkers in sepsis Blood Coagulation and Fibrinolysis 2013, 24:00–00 
• Gris JC, Cochery-Nouvellon E, Bouvier S, Jaber S, Albanese J, Constantin JM, Orban JC, Morel J, Leone M, Deras P, Elotmani L, Lavigne-Lissalde G, Lefrant JY. Clinical value of automated fibrin generation markers in patients with septic shock: a SepsiCoag ancillary study. Br J Haematol. 2018 Nov;183(4):636-647 
• (Singh N, Prasad Pati H, Tyagi S, Datt Upadhyay A, Saxena R. Evaluation of the Diagnostic Performance of Fibrin Monomer in Comparison to D-Dimer in Patients With Overt and Nonovert Disseminated Intravascular Coagulation. Clin Appl Thromb / Hemost 2015; 1-6.). 
• (Park KJ, Kwon EH, Kim HJ, Kim SH. Evaluation of the diagnostic performance of fibrin monomer in disseminated intravascular coagulation. Korean J Lab Med. 2011; 31: 143-7.). 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, weekly podcast dedicated to providing expert answers to your expert questions in coagulation.   

This week, thanks to their expert Alexandre Rousseaux, Cécile Hourquet and Audrey Carlo will question how to be more productive in the lab using a workcell.

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, the weekly podcast dedicated to providing expert answers to your expert questions in coagulation.   

This week, with our expert Lisa Gannon, product line manager, Cécile Hourquet and Audrey Carlo will question how to accurately perform an Lupus Anticoagulant diagnosis work up in the laboratory.   

Sources: 

• Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R, Derksen RH, De Groot PG, Koike T, Meroni PL, Reber G, Shoenfeld Y, Tincani A, Vlachoyiannopoulos PG, Krilis SA. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 2006; 4: 295–306 
• Devreese KMJ, Ortel TL, Pengo V, de Laat B; Subcommittee on Lupus anticoagulant/Antiphospholipid antibodies. Laboratory criteria for antiphospholipid syndrome: communication from the SSC of the ISTH. J Thromb Haemost 2018; 16: 809-813 
• CLSI Laboratory testing for the Lupus anticoagulant - Approved Guideline. CLSI document H60-A. Wayne, PA; 2014. 
• Devreese, KMJ, de Groot, PG, de Laat, B, et al. Guidance from the Scientific and Standardization Committee for lupus anticoagulant/antiphospholipid antibodies of the International Society on Thrombosis and Haemostasis. J Thromb Haemost. 2020; 18: 2828– 2839. https://doi.org/10.1111/jth.15047 
• V. Chantarangkul, F. Peyvandi, A. Tripodi, et al., Effect of different methods for outlier detection and rejection when calculating cut off values for diagnosis of lupus anticoagulants, Thrombosis Research (2018), https://doi.org/10.1016/j.thromres.2020.03.018
• CLSI Defining, Establishing and Verifying Reference Intervals in the Clinical Laboratory: Approved guideline Third edition. CLSI document C28-A3- Wayne, PA; 2008 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. 

On today’s episode, thanks to our expert Andres Ramirez, discover the part 2 of our series “how to be more productive” with a special episode about Lean and Six sigma workflow analysis.  

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.

Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. 

In our episode this week, we will discuss with François Depasse, our Clinical Development Director, about the monitoring of UFH and the appropriate tool to do it: APTT or anti-Xa activity? 

Sources:  

• Johnson EA, Mullboy B. The molecular weight range of commercial heparin preparations. Carbohydr Res 1976; 51: 119-127 
• Hirsh J, Raschke R, Heparin and Low-molecular-weight heparin : the Seventh ACCP Conference on Antithrombotic and thrombolytic therapy. CHEST 2004; 126 (suppl 3): 188S-203S. 
• Basu D, Gallus A, Hirsh J, Cade J. A prospective study of the value of monitoring heparin treatment with the activated partial thromboplastin time. N Engl J Med. 1972 Aug 17;287(7):324-7. doi: 10.1056/NEJM197208172870703. PMID: 5041701. 
• Eikelboom JW, Hirsh J. Monitoring unfractionated heparin with the aPTT: time for a fresh look. Thromb Haemost. 2006 Nov;96(5):547-52. 
• Frugé KS, Lee YR. Comparison of unfractionated heparin protocols using antifactor Xa monitoring or activated partial thrombin time monitoring. Am J Health Syst Pharm. 2015 Sep 1;72(17 Suppl 2):S90-7. 
• Vandiver JW, Vondracek TG. Antifactor Xa levels versus activated partial thromboplastin time for monitoring unfractionated heparin. Pharmacotherapy. 2012 Jun;32(6):546-58. doi: 10.1002/j.1875-9114.2011.01049.x. Epub 2012 Apr 24. 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.