Specific requirements for synthetic mRNA for different applications. https://mrna.creative-biolabs.com/6-thioguanosine-containing-cap.htm

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High throughput, simultaneous identification of multiple RBPs of interest;

Mapping specific protein-RNA complexes, such as polyclonal related RNA;

Due to ribonuclease digestion, the background of the binding site is low and the resolution is high;

No prior knowledge of RNA is required;

Whole-genome RNA screening.

https://mrna.creative-biolabs.com/rip-seq-for-rna-protein-interactions-analysis.htm

Ribonucleoprotein (RNP) complexes are formed by the binding of RNA and RBPs in cells. RBP mediates key RNA based processes, such as alternative splicing, polyadenylation, subcellular localization, translation, and miRNA regulation. Mutations in these proteins can lead to a variety of human diseases and disorders. Techniques for testing RNA protein interaction have been developed from DNA protein interaction techniques with the same principles, and RIP is one of them.