The Gemara discusses the prohibition of slaughtering an animal and its offspring on the same day (oto v'et beno). The Mishna presents various permutations of non-sacred animals (chullin) and consecrated sacrifices slaughtered either inside or outside the Temple courtyard, outlining the respective punishments incurred and the halakhic status of the meat.

A braita derives from biblical verses that this prohibition applies to consecrated animals, and the Gemara subsequently demonstrates that it applies to non-consecrated animals as well. Since the laws of oto v'et beno are learned from consecrated animals, the Gemara questions why the prohibition also applies to crossbreeds (kilayim), which are unfit for the Altar. This is resolved by explaining that the word "or" (o) in the verse serves to include crossbreeds. This explanation raises a difficulty, as the word "or" should be needed to teach that one is liable for slaughtering either an ox or a sheep with its offspring, rather than needing to slaughter both to violate the prohibition. The Gemara resolves this by pointing to the singular word "its" (oto), which teaches individual liability. Alternatively, according to Chananya - who uses the word "its" to include male parents in the prohibition - it is already self-evident that one is liable for either animal individually, in accordance with Rabbi Yonatan's interpretive principle regarding the biblical prohibition of cursing one's parents. This discussion leads to a discussion about the dispute between Chananya and the Rabbis r regarding whether the prohibition of oto v'et beno applies to both the father and mother, or exclusively to the mother.