He was born to wealthy and noble parents in Carthage (north Africa), and became a prominent lawyer in that city. Around the year 246 he embraced the Christian faith and was baptized by the priest Caecilianus. Immediately he gave all his goods to the poor and retired to a quiet place in the country to devote himself to prayer and study of Christian writings. In 248 or 249 he was elected Bishop of Carthage by the insistence of the people, though some priests opposed the consecration of such a new Christian.

Soon after his election, the Emperor Decius began a terrible persecution of Christians, during which Cyprian, in hiding, upheld his flock by letters. During this time many Christians gave in to fear of death and either sacrificed to the idols or signed statements that they had done so. When the persecution ended, the problem arose of how to treat the apostates who wished to be received back into the Church. Rigorist groups such as the Novatians and Montanists held that these lapsi had removed themselves from all hope of salvation and could never re-enter the Church. Cyprian rejected this view (as well as the position of some who would immediately reconcile the apostates); he established the position, still standard in the Church, that apostates could be restored after confession and long penance. His position led to a schism in the Church at Carthage when Cyprian's opponents set up Maximus the Montanist as a rival Bishop. The schism was only ended by a plague that swept the Empire and the city of Carthage in 253-254, together with a renewed persecution of Christians. Saint Cyprian's tireless care for the suffering during this time won most of the schismatics back to his side. When peace returned, Cyprian called a series of Councils in Carthage to resolve the conflicts that had troubled the Church. He upheld the African (and Eastern) churches' practice of reconciling heretics to the Church by Baptism rather than by laying on of hands, as was done in Rome; though Cyprian did not seek to impose this practice on other churches, Rome was not so tolerant and broke with the African church until the death of Pope Stephen.

In 256, yet another persecution broke out under the Emperor Valerian. Cyprian was arrested and brought before the Proconsul of the region. He refused to defend himself, and when told that he was to be executed, said only Deo Gratias!(Thanks be to God!). At his execution the holy bishop ordered that twenty-five gold pieces be given to the executioner, and put on the blindfold with his own hands.

Note: St Cyprian is missing on this date from traditional martyrologies because he was once confused with St Cyprian of Antioch (October 2). Today is the date of his martyrdom and the date of his commemoration on the Latin calendar.