He was the son of humble, pious parents in Phrygia, and as supported himself keeping geese. At a very early age he was granted the gift of healing illnesses of man and animals, and driving out unclean spirits.

The daughter of the Emperor Gordian (238-244) was possessed by a demon, which no physician or pagan sorcerer had been able to drive away. One day the demon shouted, 'Only Tryphon is able to drive me out!' Gordian sent servants to scour the Empire in search of the unknown healer; eventually their inquiries led them to the teenaged goose-keeper, and they brought him to Rome, where his prayers immediately drove out the demon. The Emperor showered Tryphon with gifts, which he gave away to the poor on his journey homeward.

When the persecution of Christians under Decius (250) broke out, Tryphon was denounced to the regional government as a dangerous promoter of Christianity (though he had continued to live as a humble peasant, his miracles and healings had made him known). His former service to the Emperor was either forgotten or of no account to the governor, who had him viciously tortured, then sent to Nicaea for further interrogation. There, when no torment would persuade him to deny Christ or worship the idols, he was beheaded outside the city gates. His relics were returned to Lampsacus, near his home, where he continued to work many miracles of healing.

Saint Tryphon is especially invoked for the protection of gardens and farmland against locusts, reptiles, and all small pests.