The Clinical Microbiology Procedures Handbook (CMPH) has long been a trusted resource for laboratories.  With the release of its 5th edition, this essential reference continues to guide clinical microbiologists through evolving standards, methods, and best practices.

In this episode, Luis is joined by the editors‑in‑chief, Dr. Amy Leber and Dr. Carey‑Ann Burnham, to explore what’s new in the latest edition, how the CMPH supports day‑to‑day work in the lab, and why it remains a cornerstone in clinical microbiology.

(Originally published May 4, 2023.)

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This week, Luis is joined by Dr. Christopher Doern from VCU Health to explore the complex world of diabetic wound cultures. How does diabetes impair wound healing—and what makes these infections so difficult to diagnose and treat? Dr. Doern dives into the microbiology behind diabetic foot wounds, highlighting the impact of bacterial diversity, specimen quality, and the limitations of traditional culture methods. He also shares best practices for workup strategies and antibiotic selection in these often polymicrobial infections.

Link to studies mentioned in the episode:

https://pubmed.ncbi.nlm.nih.gov/28669650/

https://doi.org/10.1111/j.1742-481X.2011.00937.x

https://academic.oup.com/ofid/article/10/10/ofad495/7292955

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Recorded live at ASM Microbe 2025, this episode captures the energy of the American Society for Microbiology’s annual event. Luis shares his top takeaways from the conference floor—including standout poster presentations, emerging technologies, and trending topics in microbiology. One clear theme dominated this year: rapid diagnostics for identification and susceptibility testing. Tune in for a front-row seat to the latest in clinical microbiology innovation.

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In this episode of Let’s Talk Micro, we dive into the fast-paced world of rapid antimicrobial susceptibility testing (AST). Recorded live from ASM Microbe 2025, Luis is joined by Dr. Carey-Ann Burnham, CEO of Pattern Bioscience and Editor-in-Chief of the ASM Case Reports journal. Together, they explore how innovative technologies—like single-cell microbiology—are transforming clinical microbiology and accelerating diagnostic results. What is single-cell microbiology, and how does it improve rapid diagnostics? They also discuss case reports from ASM that highlight the real-world impact of timely diagnostic tools on patient care. It’s where speed meets science—and the stories behind the data truly matter.

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How familiar are you with partnerships and collaborations between the laboratory and the diagnostics industry? In this episode Luis is joined by Dr. Andrea Prinzi and Dr. Kristen Smith to explore how partnerships between clinical labs, public health institutions, and the diagnostics industry drive innovation in in-vitro diagnostics (IVDs).  What is the IVD pipeline? From discovery to product launch and beyond, these collaborations improve patient care, accelerate access to new technologies, and enhance public health outcomes. Why breaking silos and fostering inclusive partnerships is essential for the future of diagnostics? Tune in to find out. 

Link to article: https://doi.org/10.1016/j.clinmicnews.2025.04.001

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TBT- This week Let's Talk Micro is talking about virology, specifically about the Human Immunodeficiency Virus (HIV). This episode features an interview with Dr. Hsu, a stem cell specialist from the Weill Medical College at Cornell University in New York. She discusses an article about a patient that has been possibly cured from HIV after a stem cell transplant with cord blood cells. What is the CCR5 gene? What is its relationship to HIV? Tune in to find out about this interesting article.

This episode was originally published on April 14th , 2022.

Link to article: 

https://news.weill.cornell.edu/news/2022/02/patient-possibly-cured-of-hiv-infection-by-special-stem-cell-transplant

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This week Luis is joined by Dr. Callan Bleick and Dr. Mohammed Al Musawa to talk about an antibiotic that was recently approved by the Food and Drug Administration (FDA): aztreonam-avibactam.  Our guests wrote a review about this drug and they joined the podcast to talk about it. What is aztreonam? What is avibactam? Why combine them? What type of activity has it demonstrated against beta-lactamase producing organisms? When will it be available? Tune in for more information.

Link to review: 

https://accpjournals.onlinelibrary.wiley.com/doi/10.1002/phar.4629

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There are many challenges when it comes to outbreak detection. It involves surveillance, time, and effort among other factors. Some of these challenges are linking multiple patients to an outbreak, or investigating a potential outbreak that turns out to be separate occurrences of an infection. In this episode Luis is joined by Dr. Alex Sundermann to talk about an outbreak detection system developed by his team at the University of Pittsburgh Medical Center. What components did they use to develop this system? What were the major outcomes? Is there a potential for laboratories to develop a system like this one?

Link to article: https://doi.org/10.1093/cid/ciaf216

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This week Luis continues going over Haemophilus. This time he goes over biochemicals, testing systems, and susceptibility testing. What is the ALA test? What commercial systems are able to identify Haemophilus? What about for Haemophilus influenzae? What is HTM agar? Are there any vaccines for H. influenzae? Tune in to find out more.

More information on  H. influenzae vaccines: 

https://www.cdc.gov/hi-disease/vaccines/index.html

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In this episode Luis continues going over organisms. This time he goes over the genus Haemophilus. We start with an overview of the different species of Haemophilus. Why can’t Haemophilus grow on blood agar? What are the X and V factors? Satellite phenomenon? What is the gram stain? What about media? Tune in for the first part of a series about Haemophilus.

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