The cloning of expressed genes involves the synthesis of DNA from mRNA using an enzyme called reverse transcriptase. Reverse transcriptase includes reverse transcriptase and a proprietary buffer designed for robust, reliable cDNA synthesis from a full range of rare and abundant transcripts. The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptase uses an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first-strand cDNA, which can be used directly as a template for the polymerase chain reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes.

There is an emerging appreciation of the existence of RNA repair pathways that rely on mRNA ligases to maintain or manipulate mRNA structure in response to purposeful mRNA breakage events. mRNA ligase is RNA repair enzymes capable of sealing breaks. The mRNA ligase can be served for joining mRNA to mRNA. Creative Biolabs offers the most extensive selection of high-quality and performance-optimized RNA ligases and masters mixes to streamline your experiments. The pre-mixed, ready-to-use formulations include a proprietary ligation reaction enhancement agent for improved performance.

RNase is an endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2',3'-cyclic phosphate, which is then hydrolyzed to the corresponding 3'-nucleoside phosphate.

mRNA polymerase is a DNA-dependent mRNA polymerase that is used for in vitro mRNA synthesis with strict specificity for its respective double-stranded promoters. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream under the promoters due to the high processivity and transcription frequency of mRNA polymerase. RNA produced using the mRNA polymerase is suitable for many applications in research and biotechnology, including radiolabeled RNA probe preparation, mRNA for in vitro translation and microinjection, RNA structure, processing, and catalysis studies, RNA amplification, and expression control via anti-sense RNA.

A series of enzymes for mRNA research, including but not limited to mRNA polymerase, RNase & inhibitors, mRNA ligase, mRNA reverse transcriptase, and mRNA capping

Anti-reverse cap analog (ARCA)-capped mRNA synthesis kit includes all the critical reagents for in vitro transcription to generate high yields of high-quality mRNA efficiently. This kit could incorporate an ARCA into the transcript. ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. The advantages of the ARCA-capped mRNA synthesis kit are that ultra-high-quality components and ARCA-based capping in correct orientation can ensure the integrity and high translation efficiency of mRNA. mRNAs synthesized with the ARCA-capped mRNA synthesis kit can be used for microinjection, cell transfection, in vitro translation, and RNA vaccines.

mRNA purification kits are designed to isolate intact messenger RNA from cells, tissues, or RNA preparations for downstream applications in the study of gene expression and transcriptome. The mRNA purification kit allows yield pure, high-quality mRNA. For instance, some kits feature all necessary reagents to remove contaminants almost completely, such as genomic DNA, ribosomal RNA, and nucleases. Due to the stringent procedure of mRNA purification, high mRNA purity can be assured for increased reliability and reproducibility in a wide range of downstream applications, such as next-generation sequencing (NGS), quantitative real-time PCR, and microarray analysis.

The mRNA isolation kit contains all necessary reagents and enough buffers for the isolation of mRNA from total RNA samples in less than one hour. mRNA isolation kits optimize the isolation of mRNA from different sample sizes or total RNA. The mRNA isolation kit allows for efficient and reproducible isolation of high-purity mRNA and permits the manual processing of multiple samples. It prepares large yields of highly purified mRNA from mammalian cells, tissue, and total RNA. The highly purified mRNA may be used directly in many molecular biology applications, such as RT-PCR, cDNA synthesis, northern blotting, northern ELISA, RNase protection assay, and even in vitro translation.

mRNA Isolation Kit

mRNA Purification Kit

mRNA has become an emerging class of gene therapy because of its negligible cytotoxicity and immunogenicity. So, there is an increasing demand for efficient and robust production of mRNA molecules in both pharmaceutics and research field. In vitro transcription (IVT) is an elegant way to obtain highly pure and uniform mRNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides.