Chrysanthos was the only son of Polemon, a prominent pagan in Rome. As befit his status, he was given every opportunity for secular learning, but seemed unable to acquire worldly wisdom. By God's providence, copies of the Gospels and the Acts of the Apostles came into his possession and, reading them avidly, he was enlightened and desired above all to be a Christian. He found a priest, Carpophorus, who instructed him in the Faith and baptised him. When his father discovered Chrysanthos' conversion, he was angry and did everything he could to turn his son back to paganism, using even threats and imprisonment. When none of these measures worked, Polemon arranged for his son to be married to a beautiful and learned young pagan woman named Daria, hoping that affection for her would draw his son away from Christ. But instead, Chrysanthus persuaded Daria of the truth of Christianity, and she was secretly baptised.

When his father died, Chrysanthus and his wife began to confess Christ openly and to live publicly as Christians. They were soon arrested and grievously tortured for their faith. The torturer, whose name was Claudius, was so moved by their endurance and patience that he himself embraced the Faith, along with his whole household. For this they were executed: Claudius by drowning, his two sons by beheading, and his wife by hanging. Finally Chrysanthus and Daria were buried alive in a pit and covered with stones. This was during the reign of the Emperor Numerian.