Join me, Cátia Moutinho, as I break down the basics of spatial transcriptomics legal battles ⚖️ between companies in this field. I'll share my opinions 💭, propose solutions 🛠️, and raise open questions ❓ that might have you thinking. This is a message for judges, companies teams and researchers. 😉

Tune in now! 🎧

The Single-Cell World Blog, Newsletter.

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In this episode, Ritika Kulshreshtha, Singleron Account Manager, tells me everything about their different kits for single cell experiments. For example, a kit to study DNA and RNA in parallel, another to study glycosylation, mRNA from yeast, or nascent RNA. So, if you are interested in doing novel single cell experiments, listen to this episode now!

Useful links:

Singleron Boot Camp

Support our Podcast (2$ per month)

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Hi  there 👋 The topic of this episode is VASA-seq, a single-cell transcriptomics method that we can use to study coding and non-coding RNA. Listen now to my conversation with Fredrik Salmén, the first author of the paper where VASA-seq is described. He gives me an amazing explanation about this method, besides his opinion on other topics related to single-cell and spatial experiments. You will not want to skip this one!

Other useful links:

* Pay us a Ko-fi

* SORT-seq

* Atrandi Biosciences

* Single Cell Discoveries

* Genevia Technologies

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In this podcast episode, I have the pleasure of talking with Kevin Travers, the Senior Vice President for R&D at LevitasBio.

Kevin tells me everything about their gentle and label-free technology that can be used during sample preparation for any single cell sequencing experiments, but also for other downstream applications.

🎧Tune in now and learn how levitation technology can make your experiments better.✨

Useful Links:

* Levitation Technology for Single Cell Genomics

* The LeviCell Systems for Cell Isolation and Enrichment

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Join us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now!

Useful Links:

* Amartya Singh LinkedIn and GitHub profile

* The starting point publication 

* TuBA paper

* Piccolo paper

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In this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multiomics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold.

Useful links:

Resolve Biosciences 

* website

* LinkedIn

* Twitter

* Youtube

* Datasets

Kieren Patel

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In this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits.

Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project!

Useful Links:

Melanie Masuda LinkedIn

Scale Biosciences(website)

* Twitter

* LinkedIn

KITS

* scDNA methylation

* scRNA

* CRISPR guide enrichment kit

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In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.

Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research. 

In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future.

Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms. 

----more----

USEFUL LINKS:

* Simone Picelli Information

* LinkedIn

* Laboratory

Publications

* SMART-Seq

* SMART-Seq 2 Original Paper

* SMART-Seq 2 Method Paper

* SMART-seq 3

* SMART-Seq 3 Express

* FLASH-seq

Protocols:

* FLASH-Seq

* FLASH-Seq UMI

* FLASH-Seq low amplification

* Bulk FLASH-Seq

Scientist.com: science as a service

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The Single-Cell World Podcast is now available in video as well, but just on Apple Podcast and in our new YouTube channel  😉

Episode 14: Single-Cell Methods, an overview, needed an update since there is a fourth method that is being used to individualize cells when using single-cell sequencing technology.

Join me and listen to it, as well as other updates concerning the methods that are at the core of the single-cell sequencing technology.

Here I leave you a gift, related with this episode.
Thank you for listening.
Kind Regards,
Cátia

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Hi there!

Today is Part 2 of the review of single-cell-related products launched in 2023. I hope I don't miss any. If I do, send me a message telling me about it. 😉

Here are some useful links:

• Exploring Single-Cell Sequencing online course

Companies that I talk during this episode (part 2):

• 
  
  • Scale Biosciences
  
  
  • Scipio Bioscience
  
  
  • Singleron Biotechnologies
  
  
  • Takara Bio
  
  
  • 10X Genomics
  
  
  • Free Resources
  
  
  • Sponsorship
  
  
  • Pay me a Ko-Fi
  
  
  
  

Thanks for listening!

Cheers,

Cátia

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe