Single-Cell World

Hi there,

Join me for an exciting episode as I talk with John Bashkin, Chief Strategy Officer of S2 Genomics, about the Singulator - an automated tissue dissociator designed specifically for single-cell experiments.

Discover how S2 Genomics is revolutionizing tissue dissociation at the single-cell level and how the Singulator enables us to get clean suspensions of cells or nuclei for our experiments. John provides fascinating insights into the Singulator's many applications, ranging from cancer research to organoids research.

Whether you're interested in exploring different approaches on tissue dissociation or simply curious about the latest developments on it, this episode is not to be missed! Tune in to learn more about Singulator technology and the future of sample preparation.

Useful Link

• S2 Genomics: Website , Twitter, LinkedIn


• John Bashkin, contact: [email protected]

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Thanks and see you in other episode.
Cátia

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Hi there,

In this episode I have a special guest, Irene Whitney the senior director of commercial strategy from Honeycomb Biotechnologies. We talk about their unique single-cell technology, the HIVE single-cell solution that allows us to study any cell, anywhere, and anytime.

She also updates me about the new versions of the HIVE system, the HIVE CLX, explaining how it works and most important, the benefits of using this technology in single-cell research compared to traditional single-cell methods.

Join me for this exciting episode as I explore the cutting-edge world of single-cell technology with Irene Whitney from Honeycomb Biotechnologies.

Useful Links:

• Honeycomb Biotechnology: Website , Twitter, LinkedIn


• Irene Whitney, contact: [email protected]


• HIVE CLX


• HIVE resources


• BeeNet Plus


• Cellometer Auto

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This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hi there,

In this podcast episode, I will tell you the difference between pooling or multiplexing samples for a single-cell experiment. I will also explain the type of tags that we can use to multiplex samples: genotype, antibodies or lipids. Finaly I will let know about the benefits of using this strategies, like single-cell cost reduction.


Here are the links for the products I mention at this episode:

• Genotyping (SNP) Arrays Examples: Illumina ; ThermoFisher


• Biolegend Antibodies & single-cell experiments


• Lipid Tags: Multi-seq (Sigma)

👉🏻 Support this Podcast!

Listen now!
Cheers,
Cátia

Don't forget to subscribe to our newsletter and blog.

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hi there,

In this podcast episode, I will tell you what three of single-cell mRNA assays we have available in order to study gene expression.

👉🏻 Whoul you like to support this Podcast?

Listen now!
Cheers,
Cátia

Don't forget to subscribe to our newsletter and blog.

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hi!
In this exciting episode, I talk with Gema Fuerte, Mission Bio's Field Application Scientist Manager for EMEA. Gema takes us on an exciting journey through the world of single-cell genomics and the revolutionary technologies developed by Mission Bio to study individual cells in unprecedented detail.

We discuss Mission Bio's goal of revolutionizing disease research at the single-cell level, as well as how their cutting-edge Tapestri platform and kits enable the detection of rare cell populations and genetic mutations that contribute to disease.

Gema provides fascinating insights into Mission Bio's technology's many applications, ranging from cancer research to translational research and clinical trials in biopharma companies and academia.

This episode is not to be missed if you are a scientist interested in exploring the frontiers of single-cell DNA analysis or simply curious about the latest developments in the life sciences. Join us as we explore Mission Bio's exciting world and the future of single-cell genomics.

MISSION BIO

• Website


• Twitter


• LinkedIn


• Gema Fuerte, contact: [email protected]

SUPPORT THIS PODCAST

• Pay me a Ko-fi

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hi there,

In this podcast episode, I will be talking about single-cell DNA. DNA is the information necessary to build our bodies. Alterations in DNA can cause changes in cell behavior, leading to diseases.

To study DNA at the single-cell level, we can use diferent protocols. Some examples are the whole-genome or the targeted approaches, the scATAC-seq or the scDNA methulation protocols.

Several commercial solutions are available and I will also mention them.

Listen now!
Cheers,
Cátia

Usefull Links

• LAST DAY!! Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023


• Support this Podcast

DNA ALTERATIONS KITS:

• REPLI-g Advanced DNA Single Cell Kit (Qiagen)


• GenomePlex® Single Cell Whole Genome Amplification Kit (Merck)


• illustra™ Single Cell GenomiPhi™ DNA Amplification Kit (Merck)


• Pico PLEX® Single Cell WGA Kit (Takara Bio)


• AMPLI1 WGA PLUS KIT (Silicon biosystems)


• MALBAC Single Cell WGA Kit (Yikon genomics)


• ResolveDNA (Bioscryb Genomics)


• scDNAseq kit (Mission Bio)

CHROMATIN ACESSIBILITY KITS:

• ICELL8 scATAC-seq (Takara Bio)


• scATAC-seq (10X Genomics)


• SureCell ATAC-Seq Library Prep Kit (Biorad)

DNA METHYLATION KITS:

• xGen™ Methylation-Sequencing DNA Library Preparation Kit (Integrated DNA Technologies)


• TWIST NGS Methylation Detection System (Twist Bioscience)


• scTAMseq(Mission Bio)

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Welcome!
In this episode I will talk about the 3 main methods that were the base for the different single-cell sequencing technology. Below you can find all companies and products links that are mentioned during this episode. I hope this is useful for your and somehow can help. Thanks for listening!

PLATE-BASED METHODS (e.g. commercial options)

• 96 well Plates (BioRad)


• REPLI-g Advanced DNA Single Cell Kit (Qiagen)


• SMART-Seq Single Cell Kit (Takara Bio)


• Evercode Whole Trancriptome (Parse Biosciences)

Micro, Nano or Pico WELL-BASED METHODS (e.g. commercial options)

• HIVE scRNAseq (Honeycomb Biotecnologies)


• GEXScope Single Cell RNA kits (Singleron)


• ICELL8 system (Takara Bio)

DROPLET-BASED METHODS (e.g. commercial options)

• Tapestri (Mission Bio)


• Chromium Controller (10X Genomics)


• Nadia (Dolomite Bio)


• DNBelab C Series (MGI)

USEFUL LINKS

• Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023


• Pay me a Ko-fi

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hi there!

In this episode I talk with Dr. Luciano Martelotto, an expert on single-nuclei experiments. When we cannot isolate happy cells for our experiments, nuclei are the next option that we have. Listen now to learn more about this topic.

Who is Dr. Luciano Martelotto?

He is A/Professor at the Head of the Development Laboratory of the Adelaide Centre for Epigenetics (ACE), South Australia ImmunoGENomics Cancer Institute (SAIGENCI). Prior to joining ACE/SAIGENCI, he was the Scientific Director of the Single Cell Laboratory at Harvard Medical School, Department of Systems Biology, Harvard University (USA). During his role at the Single Cell Lab at HMS he led a team of technology specialists in charge of developing, implementing, and offering methods of single-cell profiling to build a bridge between researchers and single-cell technologies in their quest to understand how cells collectively perform systems-level functions in healthy and diseased states.He is also a Technology Advisor for multiple companies including Omniscope, Paragon Genomics, Honeycomb Bio, ArgeTAG, Truckee Applied Genomics and South Australian Genomics Centre.

Luciano has a robust interdisciplinary scientific background, specialising in molecular biology and biochemistry with a strong background in technology and engineering. He is originally a cell and molecular biologist with a degree in Biotechnology, a PhD in Biological Sciences and Post-doctoral training in cancer genomics and single cell sequencing technologies. His diverse scientific background stems from working in a wide range of fields – plants genetics, microbiology, cancer biology and genomics – and has shaped my ambition and capacity to learn, create, develop, and apply technologies that cross multiple disciplines. The common thread woven throughout his career path has been the focus on converging ideas from various sciences and taking this ideology to develop or refine approaches to address challenges in real life settings and ultimately contribute to improving lives through technological advancements.

• email: [email protected]


• Twitter: @LGMartelotto


• LinkedIn: Luciano G. Martelotto

Usefull links

• SaltyEZ10/50 protocol


• Daughter of Frankenstein protocol


• Frankenstein Protocol


• MULTI-seq: Sigma Product & Protocol


• Job opportunity 1


• Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023


• Pay me a Ko-fi

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

In this podcast episode, I talk about different strategies that we can use when we have a lot of dead cells in our samples. Below there are links for the products and machines I mentioned on this episode.


SINGLE-CELL COURSE, May 9, 2023

Support this podcast


DEAD CELL REMOVAL KITS

• Miltenyi Biotec


• Akadeum


• Stem Cell

REAGENTS

• Ficoll-Paque


• Lymphoprep


• Histopaque


• Optiprep

MACHINES

• Luna FL


• LeviCell

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Hello there!
Season two of The Single-Cell Podcast begins today.

In this episode, I discuss the various preservation methods that can be used on samples for single-cell sequencing experiments.

Topics

• Cryopreservation


• Snap-freezing


• Fixation


• Specific single-cell strategies developed by various single-cell sequencing technologies like Singleron, 10X Genomics, Honeycomb Biotechnologies, Parse Biosciences

The course
Exploring the Cutting-Edge World of Single-Cell Technology: An Introductory Course
For more details

Pay me a kofi to support this podcast

Thank you!

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe