Single-Cell World

To celebrate that The Single-Cell World podcast has already 10 episodes, I decided to do something different. In this episode I share with you some frequent questions that I get on my talks and scientific consultations.

Besides that, tell you that this is the last episode of season 1. I will be back in 2 weeks after my holidays.

In the meanwhile, luck for your single-cell experiments!

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

What about if I tell you that you don't have to do your tissue dissociations manually?

In this episode I talk about different automated machines options that we can use to get single cells but also single nuclei. Next Thursday blog post you will find more information about this.

BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.

Gentle Mechanical Tissue Dissociators:

• gentleMACS Dissociator (Miltenyi)


• Singleron Python Tissue Dissociator (Singleron)


• Tissue Grinder (Fast Foward Discoveries)


• DSC-400 Single Cell Suspension Dissociator (RWD Life Science)


• VIA Extractor (Cytiva)


• Singulator (S2 Genomics)


• Medimachine (BD Biosciences)
  
  
  
  Thanks for listening!

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Working with solid samples?

In this episode I will talk about the different phases of solid samples preparation.

In next Thursday blog post you will find more information concerning solid samples. However I leave you here links from some of the websites and products that I mention during the podacast.

BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.

WEBSITES:

• Protocols.io


• Pubmed


• Research Gate


• bioRxiv

CELL COUNTERS

• Luna FL


• Cytosmart

Thanks for listening!

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Working with liquid samples?

In this episode I will tell you what samples were used on the published single-cell sequencing works. I will explain also the most common sample preparation methods used for these type of samples.

In next Thursday blog post you will find more information concerning liquid samples. However I leave you here links from some of the products that I mention during the podacast.

BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.

PRODUCTS:

• Strainers: FlowmiTM Cell Strainers, MACS SmartStrainers, and pluriStrainers


• Gradient Denity Solutions: Ficoll-Paque, Lymphoprep, Histopaque, and Optiprep


• Magnetic Columns: Miltenyi

Thanks for listening!

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Everyone is talking about ChatGPT! So I decided to invite it to my podcast.

Are you curious to know its knowledge of single-cell research and technologies? Listen now 😉

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

In this episode, I'll go over the different types of samples that can be used in single-cell sequencing experiments.

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

In this episode I talk about the checklist that I use while planning single-cell experiments. Basically are the essential questions that when answered help me deciding the best approach for the project.

FREE GIFT: Single-Cell Planning Checklist

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

Sooner or later, you would like to do a single-cell experiment. In this episode I explain you the key terms that you should know in order to avoid misunderstandings while planning your future experiment or project. E.g. libraries, UMI, reads...

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

In this episode I will tell you what are the most common biological topics/questions that researchers want to answer using single-cell technology. They are just 3!

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

It seems that nowadays single-cell experiments are a must-have in all high-impact publications. But what is this technology?

In this first episode I will explain you what is single-cell and how it differs from the standard experiments.

Find other FREE resources on www.thesinglecellworld.com
I would love to know more about you. Follow me @singlecell_world (Instagram or Twitter).

This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe