'Saint Barsanuphius the Great, who was from Egypt, and his disciple, Saint John the Prophet, struggled in very strict reclusion during the sixth century at the monastery of Abba Seridus at Gaza of Palestine, and were endowed with amazing gifts of prophecy and spiritual discernment. They are mentioned by Saint Dorotheus of Gaza, their disciple, in his writings. Many of the counsels they sent to Christians who wrote to them are preserved in the book which bears their names. Once certain of the Fathers besought Saint Barsanuphius to pray that God stay His wrath and spare the world. Saint Barsanuphius wrote back that there were "three men perfect before God," whose prayers met at the throne of God and protected the whole world; to them it had been revealed that the wrath of God would not last long. These three, he said, were "John of Rome, Elias of Corinth, and another in the diocese of Jerusalem," concealing the name of the last, since it was himself.' (Great Horologion)

Saint Barsanuphius lived in such reclusion that only Abbot Seridus ever saw him: once a week the Abbot would bring him three loaves and some water, and would write down the Saint's counsels. Some of the brethren came to suspect that Barsanuphius was an invention of the Abbot, and to relieve their minds he came out of his cell for the only time, greeted them, washed their feet, and withdrew again.

It is unknown when St Barsanuphius reposed. When it was suspected that he had died in his cell, the Patriarch of Jerusalem ordered that it be opened, but fire blasted forth from the door, preventing any from entering.