This in-depth essay explores Leonardo da Vinci’s The Last Supper, tracing its creation, symbolism, technical innovations, and historical context within the vibrant court of Ludovico Sforza in Renaissance Milan. It analyzes the revolutionary use of perspective, emotional expression, and subtle Eucharistic symbolism that set the work apart from earlier Last Supper depictions. It also examines the painting’s deterioration due to Leonardo’s experimental technique, its restoration efforts, and its lasting impact on art history. Comparisons with Leonardo’s other masterpieces like the Mona Lisa and the Vitruvian Man reveal a coherent vision blending science, faith, and human psychology, solidifying The Last Supper as one of the most iconic works of Western art.