In Part 2 on 5-Amino-1MQ (5A1MQ), we get practical and responsible: how to think like a researcher, how to interpret viral before/afters without getting played, what biomarkers actually matter, and what “oral dosing” patterns are commonly claimed online as of 2026—with a clear reminder that this is not a standardized, published human dosing regimen.

This episode is NOT medical advice. 5-Amino-1MQ is a research compound and is not FDA-approved to treat or prevent any condition. If you suspect metabolic disease, celiac disease, or you’re on medications, talk to a qualified clinician.

In this episode:

What a smart, ethical research mindset looks like

How to analyze online claims (water weight vs fat loss, confounders, placebo, “without changing anything”)

What to track if human trials begin reporting (body composition, glucose/insulin, lipids, liver markers, inflammation)

The “mechanism markers” researchers care about (NNMT pathway / metabolites)

Safety signals & red flags worth watching (sleep, GI, mood, labs)

“Oral dosing” trends that are advertised/reported online in 2026 (not a recommendation)

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#5A1MQ #5Amino1MQ #NNMT #NADPlus #MetabolicHealth #FatLossScience #Biohacking #Longevity #InsulinResistance #BloodSugar #FattyLiver #Sarcopenia #Mitochondria #HumanPerformance #TheHuman50Project

Is 5-Amino-1MQ (5A1MQ) the “fat-loss NAD+ compound” people claim—or is it just early-stage science getting overhyped? In this episode, JP (UltimateBiohacker10x / The Human 5.0 Project) breaks down what 5A1MQ actually is, how NNMT inhibition works, what the best animal data shows for obesity and fatty liver, what we’re seeing in aged muscle performance studies, and the most important part: what we still do NOT know in humans as of January 2026.

Key sources (for show notes):

NNMT inhibitor 5A1MQ cell/NAD+ findings (Neelakantan et al., 2017). PMC

DIO mouse obesity + fatty liver pathology + PK (Babula et al., 2024, Diabetes Obes Metab). PMC

Aged mice muscle function improvements with NNMT inhibition (Dimet-Wiley et al., 2024). PubMed+1

Human NNMT/MNA association with insulin resistance/T2D (Kannt et al., 2015). PMC

NNMT + NAD+ / aging review context (Li et al., 2024). PMC

Disclaimer: Educational content only. Not medical advice.

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#5A1MQ #5Amino1MQ #NNMT #NAD #NADPlus #MetabolicHealth #FatLossScience #Longevity #Biohacking #Mitochondria #InsulinResistance #FattyLiver #Sarcopenia #HumanPerformance #Ultimatebiohacker10x

Welcome to The Human 5.0 Project with UltimateBioHacker10X.

Gluten has become the internet’s favorite scapegoat—but here’s the weird part: people who feel terrible after bread in the U.S. often report feeling totally fine eating bread in France, Italy, or Spain. Same person… different bread. So what’s really going on?

In this episode, we unpack:

Why US flour processing can be radically different (bleaching, enrichment, shelf-life priorities)

How long fermentation / sourdough-style methods may change digestibility

The role of additives & dough conditioners (and why ingredient lists matter)

Why some “gluten sensitivity” may actually be wheat components, FODMAPs, additives, or gut irritation—not gluten itself

Who should never “test it” casually (celiac disease + true wheat allergy)

Important: This podcast is for education only, not medical advice. If you suspect celiac disease or a wheat allergy, talk to a qualified clinician before changing your diet or “trialing” gluten.

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

If you want Part 2 (best bread choices + how to run a real elimination/reintroduction test without guesswork), Click here!

#Gluten #GlutenFree #Bread #GutHealth #Digestion #FoodScience #Sourdough #FODMAP #Wheat #Nutrition #Biohacking #Longevity #FoodAdditives #TheHuman50Project #UltimateBioHacker10X

Welcome back to The Human 5.0 Project with UltimateBioHacker10X

In Part 2 on SLU-PP-332, we skip the hype and talk side effects and the biggest online rumor: cardiomyopathy / heart risk. We break down what people commonly report anecdotally (like flushing, water retention, and fatigue when dosing too aggressively) and then zoom out to what preclinical mouse data can—and can’t—tell us about cardiac safety (histology, fibrosis signals, and functional echo-style outcomes).

Important: SLU-PP-332 is a preclinical research compound and is not approved for human use. This content is for education only and is not medical advice.

⏱️ In this episode:

Commonly reported “human chatter” side effects (anecdotes vs evidence)

The cardiomyopathy rumor: what preclinical endpoints look for

Heart markers and organ-system signals people reference (and limitations)

The real bottom line: what we know vs what we still don’t (humans)

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#SLUPP332 #SLUPP332SideEffects #Peptides #Biohacking #Longevity #HumanPerformance #Mitochondria #ExerciseMimetic #Cardiomyopathy #HeartHealth #PreclinicalResearch #MouseStudies #WellnessScience #TheHuman50Project #UltimateBioHacker10X

Most people chase growth hormone peptides without understanding how the pituitary actually works — and that’s how you end up with hormone shutdown, stalled gains, or long-term damage.

In this episode of The Human 5.0 Project, Ultimate Biohacker 10X breaks down growth hormone–releasing peptides in plain English using a simple “phone call” analogy so you finally understand what these compounds actually do inside your body.

We cover:

If you’ve ever used — or considered using — growth hormone peptides for fat loss, muscle growth, recovery, or longevity, this episode explains why less is more and how to avoid long-term hormonal damage.

Download the FREE GH Peptide Cheat Sheet

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

Comment below: Which peptide are you currently using — or considering?

Subscribe for weekly deep dives into peptides, hormones, longevity, and regenerative medicine.

Stay hacked. Stay curious. Stay hormonally intelligent.

#GrowthHormone#GHPeptides#Ipamorelin#CJC1295#Tesamorelin#Sermorelin#IGF1#PEGMGF#PeptideTherapy#HormoneOptimization#PituitaryHealth#EndocrineSystem#MuscleGrowth#FatLoss#Longevity#Biohacking#Human50Project#UltimateBiohacker10X

Your pituitary gland and HPA axis control nearly every hormonal signal in your body — from stress and metabolism to growth, reproduction, and long-term healthspan.

In this episode of The Human 5.0 Project, Ultimate Biohacker 10X delivers a clear, clinician-grade breakdown of how the pituitary gland actually works — without fluff or fear-mongering.

You’ll learn:

This episode connects neuroscience, endocrinology, and real-world healthspan into one integrated framework.

 Download the full hormone signaling map & HPA axis PDF:

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

Subscribe for weekly deep dives into hormones, longevity, peptides, and performance.

Comment below: What hormone system do you want decoded next?

#PituitaryGland#HPAAxis#HormoneHealth#Endocrinology#StressHormones#Cortisol#GrowthHormone#ThyroidHealth#HormoneOptimization#Biohacking#Longevity#Healthspan#FunctionalMedicine#Human50Project#UltimateBiohacker10X

The Gut Microbiome Explained: Why Your Health Starts in Your Intestines

Your gut microbiome isn’t just about digestion — it’s a living ecosystem of over 38 trillion microorganisms that regulate nearly every system in your body. From hormones and immunity to inflammation, metabolism, and even mood, your gut bacteria quietly shape your health from the inside out.

In this episode, we take a deep dive into the intestinal microbiome (oral microbiome coming next) and break down what the science actually shows.

Inside this episode:

If you’re dealing with bloating, fatigue, hormone chaos, autoimmune symptoms, or unexplained inflammation — your gut ecosystem may be the missing link.

🎯 Download the free Gut Microbiome Reset PDF

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#GutMicrobiome #GutHealth #MicrobiomeScience #LeakyGut #IBD #AutoimmuneHealth #HormoneBalance #Butyrate #Akkermansia #Faecalibacterium #DigestiveHealth #Biohacking #Longevity #FunctionalMedicine #Human50Project #UltimateBiohacker10X

Methylation Explained: Testing, MTHFR, Methylated Vitamins & the Gut-Brain Connection

Ever feel like low energy, mood swings, or brain fog is holding you back? It might be methylation – the body's core biochemical process for gene regulation, detox, and neurotransmitter balance. In this 12-minute episode, we break down what methylation is, how to test for deficiency (MTHFR & homocysteine), if testing even matters, why methylated vitamins (like methylfolate & methyl-B12) outperform regular forms for many, and the gut's surprising role (bacteria in yogurt don't methylate, but microbiome influences epigenetics via B vitamins & metabolites).

Key takeaways:

• Methylation basics: Adding CH3 groups to DNA/proteins – essential for healthspan

• Deficiency signs & testing: MTHFR gene variants, homocysteine levels (goal <9 µmol/L)

• Methylated vitamins: Benefits for MTHFR folks (better absorption, no masking B12 deficiency)

• Gut connection: Bacteria produce B12 (methylation cofactor); microbiome shapes DNA methylation patterns

• Protocols: Supplements, diet tweaks, when to test

If you're optimizing for longevity, mood, or detox – this is your primer.

Download the free Methylation Cheat Sheet (testing guide, supplement doses, gut tips)

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#Methylation #MTHFR #MethylatedVitamins #Methylfolate #MethylB12 #GutMicrobiome #Epigenetics #Homocysteine #DetoxPathways #MoodBoost #EnergyHack #Longevity #Biohacking #FunctionalMedicine #VitaminDeficiency #BVitamins #Human50Project #UltimateBiohacker10X

The peptide revolution is here — and it’s changing how elite athletes, biohackers, and longevity clinicians approach healing, fat loss, and performance.

In this episode of The Human 5.0 Project, we break down the Top 10 Most Powerful Peptides of 2025, based on real-world use, clinical data, and years of hands-on experience — not hype.

You’ll learn what actually works, what’s overrated, and how advanced protocols are being used responsibly to optimize recovery, metabolism, and long-term health.

• BPC-157 & TB-500 – The “Wolverine Stack” for tendon, ligament, and gut repair

We also cover:

📘 Free Download:

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#Peptides2025 #BPC157 #TB500 #WolverineStack #Semaglutide #Tirzepatide #Retatrutide #MOTSc #KPV #CJC1295 #Ipamorelin #IGF1LR3 #PeptideTherapy #Biohacking #RegenerativeMedicine #MuscleRecovery #GutHealing #WeightLossPeptides #LongevityPeptides #Human50Project #UltimateBiohacker10X

Microglia Are Controlling Your Anxiety — The Brain’s Hidden Immune Switch

What if anxiety, OCD, and chronic stress aren’t just “mental” — but immune disorders of the brain?

In this episode of The Human 5.0 Project, we break down groundbreaking 2025 research revealing that microglia — the brain’s immune cells — act as biological “brakes” or “accelerators” for anxiety and compulsive behavior. When these cells malfunction, anxiety and obsessive behaviors can spiral out of control.

Drawing from landmark work by Nobel laureate Mario Capecchi, this episode explores how Hoxb8 microglia regulate emotional behavior — and how dysfunction in this system may explain chronic anxiety, OCD, and stress-related disorders.

• Why anxiety may be driven by immune dysfunction, not weakness

This is the future of mental health — not suppressing symptoms, but restoring balance at the cellular level.

PDF of Show with Lifestyle Hacks(Microglia and Mental Health)

For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.

And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.

#Microglia #AnxietyScience #Neuroinflammation #MentalHealthResearch