Sequence elements mediating binding to proteins involved in mRNA trafficking and translation

Sequences repressing deadenylation of mRNA

https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

Regulatory sequence elements binding to molecules involved in mRNA trafficking and translation

Sequences inhibiting-exonucleolytic degradation

https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

Poly(A) tail:

Masked/unmasked poly(A) tail affecting translation

Length of poly(A) tail affecting stability

Modified nucleotides inhibiting deadenylation

https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

Optimization Service to Improve mRNA Translation and Stability

Coding region:

Optimized codon usage to improve the translation

Optimized base usage to reduce the endonucleolytic attack

https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

Optimization Service to Improve mRNA Translation and Stability

5'-cap modifications:

Uncapped, functional when combined with IRES (internal ribosome entry site)

Cap analogues mediating binding to eIF4E (eukaryotic translation initiation factor 4E)

Cap analogues conferring resistance to decapping

The concept behind using IVT mRNA as a drug is the transfer of a defined genetic message into the cells for the ultimate purpose of preventing or altering a particular disease state. One method is to transfer mRNA into the patient's cells ex vivo. These transfected cells are then adoptively administered back to the patient.https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

In vitro transcribed (IVT) messenger RNA (mRNA) has recently come into focus as a potential new drug class to deliver genetic information. Such synthetic mRNA can be engineered to transiently express proteins by structurally resembling natural mRNA. https://mrna.creative-biolabs.com/mrna-pharmacology-optimization.htm

RNA methylation assay https://mrna.creative-biolabs.com/rna-methylation-assay.htm

RNA Fluorescence Assay https://mrna.creative-biolabs.com/rna-fluorescence-assay.htm

RNA methylation assay https://mrna.creative-biolabs.com/rna-methylation-assay.htm