In the fifth episode of the Longevity & Aging Series, Dr. Amit Sharma, Group Lead from SENS Research Foundation, Mountain View, CA, discusses a research paper he co-authored that was published in Volume 14, Issue 5, of Aging (Aging-US), entitled, “Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells.”

DOI - https://doi.org/10.18632/aging.203931

Corresponding Author - Amit Sharma - [email protected]

Video - https://www.youtube.com/watch?v=WQR8_gm2gUI

Abstract

In the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have emerged as promising alternatives to drug-based therapeutic interventions for the prevention and treatment of age-related disease and debility. Given the promise of NK cell-mediated immunotherapies as a safe and effective treatment strategy, we outline an improved method by which primary NK cells can be efficiently enriched from human peripheral blood across multiple donors (ages 20-42 years old), with a practical protocol that reliably enhances both CD56dim and CD56bright NK cells by 15-fold and 3-fold, respectively. Importantly, we show that our co-culture protocol can be used as an easily adaptable tool to assess highly efficient and selective killing of senescent cells by primary NK cells enriched via our method using longer co-culture durations and a low target to effector ratio, which may be more physiological than has been achieved in previous literature.

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Longevity & Aging Series

Aging (Aging-US) and FOXO Technologies have teamed up for a special collaboration on aging research with a monthly video series: Longevity & Aging Series. This series invites Aging researchers to speak with host Dr. Brian Chen, an adjunct faculty member at the University of California San Diego and Chief Science Officer of FOXO Technologies.

Learn more - https://www.aging-us.com/longevity

Keywords - aging, senescence, natural killer cells, NKCC, immune surveillance

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

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