This Week in Virology, the podcast about viruses, celebrated its 300th episode on Tuesday, August 26, 2014 with a live recording at the Washington, DC headquarters of the American Society for Microbiology. This special episode features the TWiV hosts Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler recording together in person for the first time.