Aging published this trending paper on March 2, 2020, entitled, “ESHRD: deconvolution of brain homogenate RNA expression data to identify cell-type-specific alterations in Alzheimer’s disease” by researchers from Neurogenomics Division, The Translational Genomics Research Institute, Phoenix; Center for Statistical Genetics, Department of Neurology, Gertrude H. Sergievsky Center, Columbia University Medical Center, New York; The University of Sydney School of Medicine, Sydney; University of Miami; Banner Sun Health Research Institute, Sun City.

The researchers conducted brain region cell-specific pathway analysis and Gene Set Enrichment Analysis (GSEA). The team mapped and measured five different cell types in seven different brain regions. The cell types included: microglia, neuron, endothelial, astrocyte, and oligodendrocyte. Endothelial and oligodendrocyte are two cell types that are not easily examined in the brain and only very little gene expression data previously existed for Alzheimer’s disease.

“We conducted RNA expression profiling from both brain homogenates and oligodendrocytes obtained by LCM from the same donor brains and then calculated differential expression.”

The researchers used a dataset of Multiple System Atrophy (MSA) patients (n = 4) and controls (n = 5) to validate their ESHRD method. Homogenate, LCM, and scRNA-Seq results were compared using the ESHRD method. They also compared their findings to other research studies.

To date, this study has generated an Altmetric Attention Score of 10. The Altmetric Attention Score provides an at-a-glance indication of the volume and type of online attention the research has received.

Top Aging publications rated by Altmetric score - https://www.aging-us.com/news_room/altmetric

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DOI - https://doi.org/10.18632/aging.102840

Full text - https://www.aging-us.com/article/102840/text

Correspondence to: Matthew J. Huentelman email: [email protected]

Keywords: RNA sequencing, laser capture microdissection, brain homogenates, endothelial cells, oligodendrocytes, Alzheimer’s disease

About Aging

Launched in 2009, Aging publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

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