Even though the basin in the Temple must be large enough for four people to simultaneously wash their hands and feet from it, a kohen may also perform this washing using a smaller utensil - provided the water originates from the basin and the utensil is sanctified (a kli sharet).

Reish Lakish ruled that a liquid suitable for completing the required volume of a mikveh may also be used to complete the volume of water in the Temple basin. However, such a liquid is not valid for the quarter-log amount required for netilat yadayim (ritual handwashing). The Gemara explores what types of liquids are excluded from use in netilat yadayim. Initially, it suggests excluding liquefied clay or aquatic organisms like red gnats, which are considered water-like. Both suggestions are ultimately rejected.

Instead, the Gemara concludes that the exclusion applies to a case where one adds a se'ah of liquid to a mikveh that contains exactly forty se'ah, then removes a se'ah, repeating this process until half the mikveh consists of the added liquid. This method is acceptable for a mikveh and the Temple basin, but not for the quarter-log required for handwashing. Rav Papa introduces a unique case where such a liquid would be valid for tevilah (immersion) of very small items.

Rabbi Yirmia, quoting Reish Lakish, stated that water from a mikveh may be used in the Temple basin. This raises a question: perhaps the basin requires flowing water rather than stagnant water. Although a tannaitic source seems to support this requirement, the Gemara resolves the issue by showing that it is a matter of dispute among the tannaim.

The Mishna teaches that if an uncircumcised kohen performs sacrificial service in the Temple, the sacrifices are disqualified. This ruling is derived from Yechezkel 44:7,9.

Similarly, a kohen who is impure disqualifies the sacrifices he offers. The elders of the South limit this disqualification to impurity from a sheretz (creeping creature), but not to impurity from contact with the dead, which is permitted when the majority of the community is impure.

The Gemara challenges this view, noting that impurity from the dead is more severe as it lasts seven days and requires purification through the ashes of the red heifer. However, the elders argue that since communal sacrifices are accepted when the majority are impure from the dead but not from a sheretz, the same distinction applies to kohanim: a kohen's sacrifice is not disqualified if he is impure from the dead.

To better understand the elders' position, the Gemara concludes that they must hold that someone impure from a sheretz on the 14th of Nissan may have the Paschal sacrifice offered on their behalf and eat it on the night of the 15th in a state of purity.

Ulla explained that Reish Lakish strongly disagreed with the elders of the South. He argued that the laws governing the community are more lenient than those governing the kohanim. While the people may have their Paschal offering brought on their behalf when impure, a kohen's offering is disqualified if he is impure from a sheretz. Therefore, if the Paschal offering cannot be brought on behalf of someone impure from the dead, then certainly a kohen who is impure from the dead should disqualify the sacrifice he offers.

To resolve Reish Lakish's difficulty, the Gemara suggests that the elders of the South may have held that even the Paschal offering could be brought on behalf of someone impure from the dead. This raises a question: how does this view align with the laws of Pesach Sheni?