Title: SBU PROS

Description: There is currently no therapy to administer solely protein S directly into the bloodstream to treat protein S deficiencies among other serious disorders. We are Stony Brook University’s 2022 iGEM team, and our project, PROS, proposes an injectable delivery of a recombinant human protein S therapy. Through our project, we hope to address current gaps in treatment and existing health disparities.

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Title: Less oxygen, more possibilities

Description: Our project stems from the idea of biological hydrogen production and nitrogen fixation. It is found that hydrogenase and nitrogenase have the oxygen paradox. In response to this problem, we propose to construct a system called Oxygen Hunter to maintain an intracellular hypoxic environment and normal metabolism. Therefore, leg hemoglobin was selected to enrich oxygen to the respiratory site, and laccase catalyzed oxygen reduction to water. We decided to compare its effect, so we chose an anaerobic promoter-connected fluorescent protein to detect oxygen concentration and oxygen consumption rate. We hope to introduce application components for the biological production of reducing products, combined nitrogen fixation, hydrogen production, and reduction of organic matter and carbon dioxide, and can provide a low oxygen environment to promote the reduction of biomass as energy products.

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Title: A Novel Therapeutic, Modified Dressing- With The Functional PQQ

Description: Our project is to use Bacillus subtilis natto as a host to achieve higher efficiency in the production of PQQ so that the yield can be high enough for commercial products and also develop wound dressings with PQQ in hope of reducing the risk of microbial infection in diabetic patients after the wound is formed.

This video briefly introduced what PQQ is （Pyrroloquinoline quinone ）,why we want to utilize PQQ in diabetics wound dressing and why we choose Bacillus subtilis natto as a host to produce PQQ.

#diabetes

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Title: Recombinant spider silk gels as bio-ink for 3D printing of artificial skin

Description: Existing methods of making artificial skin have some drawbacks. Some of the materials used in artificial skin can be rejected. Also, the material's physical properties, such as flexibility, are insufficient to support complex medical needs. In addition, the production of artificial skin also faces the problem of complex production process and high cost. Therefore, we expect to develop an artificial skin with high preparation efficiency, low cost, low rejection, and high flexibility to solve these problems.

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Title: To Drink Or Not To Drink

Description: Lactose intolerance indicates intestinal discomfort caused by decreased beta-galactosidase to digest lactose. Since lactase hydrolyses lactose into glucose and galactose, when the expression of lactase declines due to genetic disorder or natural ageing, the amount of lactose people can tolerate will decrease. It is a worldwide health problem that about 70% of the world population suffered from lactose intolerance. Notably, the incidence is particularly high in Asia because the downward trend of expressing lactase is strengthened by diets rarely containing lactose. In the dairy industry, Aspergillus oryzae is one of the most widely used fungal sources of beta-galactosidase (lacA) to produce lactose-free products for people with lactose intolerance. To allow people with lactose intolerance to enjoy dairy products, our project aims to express lacA heterologously in Pichia pastoris, quantify the enzymatic property of lacA, and optimise the culture conditions of recombined Pichia pastors.

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Title: S. aureus Bacterial Inhibition with TurboID

Description: During community communications, we often hear about a pathogenic bacterium, Staphylococcus aureus. Our main goal is to use protein proximity labeling to make S.aureus harmless within vivo. TurboID can catalyze biotin and ATP it carried to produce Biotinyl-5'-AMP, and label the lysine that are approximate to the active site. After labeling, the bacteria will not be able to activate the gene of virulence production. Such process can be accomplished within ten minutes in 25℃, which is the most efficient and reachable method by far. This method will not only be able to inhibit Staphylococcus aureus but it can also target other bacteria with further research.

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Title: Curli Capture: Engineering bacterial biofilms to reduce nitrate pollution.

Description: Nitrate runoff, or the loss of nitrate nitrogen from agricultural systems, can lead to issues such as unsafe drinking water and overgrowth of plant life. To combat this issue, we have designed a nitrate-capture system utilizing curli proteins, which are self-assembling amyloid fibers secreted by E. coli that will form a mesh-like structure upon secretion. Our goal is to fuse the nitrate-capturing protein native to cyanobacteria, nrtA, to the curli monomeric unit, csgA. This fusion will be done with the SpyTag/SpyCatcher system, a two-part system that, upon contact, spontaneously forms an isopeptide bond. We are planning our capture system to be a cell-free mesh that can be encapsulated and placed near waterways in agricultural communities. However, we eventually want to modify our construct into a cellular system under the control of an inducible promoter that produces nitrate-capturing curli only in the presence of nitrate.

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Title: Cultured Meat

Description: We will simulate the environment of cells in vivo, and use E.coli with independent dynamic regulation system to synthesize polyhydroxyl fatty acids with superior performanceas the scaffold material to make porous microspheres to promote the flesh-forming growth of animal muscle cells in vitro. And add collagen to restore meat more authentic taste.

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Title: NanoBlade

Description: We are Team ELTE, and we have created NanoBlade, which could facilitate both tumor diagnostics and therapy! We engineered E. coli to selectively attach to cancer cells and colonize tumor tissues. These interactions are detectable via fluorescence, so with the help of the bacteria, tumors can be visualized. Under blue light, our bacteria release toxins into their environment, killing cancer cells locally.

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Title: Bacteria-Battling Bombolitin

Description: Ralstonia causes bacterial wilt in Solanaceous crops worldwide. In an attempt to combat this problem, farmers use pesticides. However, pesticides result in detrimental effects on the environment, consumers, and farmers. Our project aims to find an alternative to these harmful chemicals. By using a novel expression system in Escherichia coli, we can increase the production of bombolitin, an antimicrobial peptide that inhibits Ralstonia, with lowered cost and difficulty.

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