In this episode of Speaking of Mol Bio, Aistė Serapinaite, an experienced R&D scientist, shares her insights into the world of 1-Step RT-PCR—a method that simplifies RNA analysis by combining reverse transcription and PCR amplification in a single reaction. She explains how traditional RNA workflows once lengthy, multi-step processes were prone to errors and contamination, and how 1-Step RT-PCR has transformed this landscape with speed, efficiency, and fewer handling steps.

Listeners learn about the technical workings of 1-Step RT-PCR, including the importance of primer design, RNA quality, and essential controls to ensure reliable results. Aistė highlights the Invitrogen SuperScript IV UniPrime One-Step RT-PCR System, emphasizing features like universal annealing temperatures and high sensitivity, capable of detecting even trace levels of RNA.

The episode also explores diverse applications, from gene expression studies and cancer biomarker detection to monitoring viral pathogens such as SARS-CoV-2 and Zika virus. While acknowledging the limitations of 1-Step RT-PCR—like the inability to archive cDNA for future assays—Aistė affirms its role as a fast, robust, and eco-friendly solution for high-throughput molecular biology labs. Whether you're new to molecular workflows or an experienced researcher, this episode offers valuable tips and tools to optimize your RNA experiments.

Helpful resource links mentioned in this episode:

• See how one-step RT-PCR is used for amplicon-based viral genome sequencing
• View a video on the differences between one-step and two-step RT-PCR
• Access the Oligo Perfect Primer Designer tool
• Order or check out the brochure for Invitrogen SuperScript IV UniPrime One-Step RT-PCR System
• Order Invitrogen ezDNase Enzyme

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Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. 

For Research Use Only. Not for use in diagnostic procedures.