Dr. Hansen’s lab conducted several in vitro experiments where choline chloride was supplemented to beef embryo culture media for the first seven days of embryonic development. Calves resulting from the choline-supplemented embryos were consistently 17-20 kilograms heavier at weaning. In the feeding experiment presented at the Florida Ruminant Nutrition Symposium, Dr. Sagheer fed rumen-protected choline to beef cows one day before AI through seven days post-AI, spanning ovulation, fertilization, and the first seven days of embryo development. In contrast to the in vitro studies, calves born to cows supplemented with choline during the peri-conception period were lighter at weaning than control calves. The panel discusses potential mechanisms of action for these results, including choline’s role as a methyl donor potentially impacting the epigenetic programming of the embryo.