My Longevity Experiment

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Please note that the links above are affiliate links, which means that if you use the code, I will receive a small commission, and if you do, may I say many thanks I advance for your support.

My Current Anti-Aging Protocol:

·      1.5 grams of an NADBooster (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

·      5 grams of Creatine (1 month on, 2 months off)

DISCLAIMER: The Video Content has been made available for informational and educational purposes only. The video creator does not make any representation or warranties with respect to the accuracy, applicability, fitness, or completeness of the Video Content. The Video Content is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of your physician or other qualified health provider with any questions you may have regarding a medical condition. The video creator hereby disclaims any and all liability to any party for any direct, indirect, implied, punitive, special, incidental or other consequential damages arising directly or indirectly from any use of the Video Content, which is provided as is, and without warranties.

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How to live healthy, Live to 100, Human mortality study, Life expectancy, Human Life expectancy, maximum human lifespan, antiaging, Longevity, Mortality, Longevity study, lifespan study, what is maximum human lifespan, what is human lifespan, what is Healthspan, Lifespan, Healthspan, longevity diet, longevity secrets, longevity research, longevity exercises, longevity supplements, antiaging tips, antiaging secrets, antiaging podcast, how to live longer, how to live healthy.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

 Signal To Noise by Scott Buckley https://soundcloud.com/scottbuckley

Creative Commons — Attribution 3.0 Unported — CC BY 3.0

Free Download / Stream: https://bit.ly/signal-to-noise

Music promoted by Audio Library https://youtu.be/of0Ei_T4isc

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

Renue by Science 10% Discount Code: MYNMN (https://renuebyscience.com/?rfsn=5692699.331801&coupon-code=MYNMN)

Renue by Science 15% Subscription Service Discount Code: MYNMNSUB

Youth & Earth 15% Discount Code: MYNMN (https://youthandearth.com?sca_ref=3766764.knrWl4O3cB)

ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

Please note that the links above are affiliate links, which means that if you use the code, I will receive a small commission, and if you do, may I say many thanks I advance for your support.

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Why is deep sleep such a big deal, as long as you get around 7 hours total sleep a night, shouldn’t that be enough?

Deep sleep has an ancient, restorative power to clear waste from the brain, it’s our body’s way of taking out the trash; waste clearance occurs whilst we are awake but is substantially enhanced during deep sleep.

This waste potentially includes toxic proteins that may lead to neuro-degenerative diseases such as Alzheimer's disease and Parkinson's.

My Sleep Statistics 2019

You can see here that my sleep on 29th, 30th and 31st of October 2019 was good as far as overall time is concerned, 7:34, 7:49 and 7:10.

But my deep sleep; the most rejuvenating sleep only accounted for 8% and 17% of the overall total.

I managed to increase that to over 25% and if some cases over 40%, and now I will tell you how.

Fitness Tracker

As part of my NMN Experiment I purchased a fitness tracker to monitor my heart rate, another function of this tracker is a sleep monitor, it records overall sleep, light sleep, deep sleep and minutes awake.

Link below to the one I use...I may do a sperate video on it, let me know if you’d like that.

I Experimented with different sleep aids, techniques, types of food, when I ate food, alcohol intake etc.

Unless there is an unexpected occurrence, I can now pretty much guarantee that I will get 7 to 8 hours of sleep a night, but more importantly that over 20%, (in one case 43%) will be deep, rejuvenating sleep.

SMART Lights

With white, blue and green light being such an issue to our alertness and melatonin levels I stared to research smart light bulbs, bulbs that I could programme to dim and gradually turn red 2 hours before I actually went to bed.

When the lights are on after 8pm it reminds me of the Ops Rooms that I would often frequent when I was in the Army…no white light after sundown.

I used these in my study, the living room and the bedroom, in conjunction with my blue light blocking glasses (which I will cover later).

Link Below to the bulbs that I use, you will need to download an app so you can control them all from your phone.

Blue and Green Light Blocking Glasses

I listened to the likes of Dave Asprey who extoled the virtues of Blue and green light blocking eyewear, of which he owns a company,

I also watched an interview with the BLUblox CEO, Andy Mant where he further explained the science behind how blue light affects our sleep patterns.

The science seemed unquestionable, so I purchased this pair of glasses.

Did they work, well when you put them on 2 hours before you sleep, they certainly do make you feel sleepy.

However, over a period of a few months I tried wearing them on and off and made sure that all lights were out before I went to bed and only had the red lights on if I had to go to the toilet during the night.

They made absolutely no difference to my overall sleep, but more importantly to my deep sleep. This may be different for you, Dave Asprey or Andy Mant, but for me ZERO effect!

So, they are now in the cupboard of broken dreams.

Eye Mask

The next thing I tried was an eye mask; I bought this one from Amazon.

Again I tried this with

·     The SMART lights and nothing else

·     The blue light blocking glasses and nothing else

·     The smart lights and the glasses

·     And with just the eye mask and bright white light until I got into bed

This did improve my deep sleep considerably and it allowed me to walk around the house, watch TV and use my smart phone without having to wear the blue light blocking glasses.

So, this $13 eye mask is now a consistent part of my nighty routine.

Blackout Curtains

I had blackout curtains in our bedroom, but there was still a certain amount of light that bled in, this is because there were 2 very large floor to ceiling windows in the bedroom.

When we moved to a new house that had 1 smaller window that was completely covered by the curtains, again my deep sleep improved.

I think that the Blackout curtains and blackout eye-mask are the biggest contributors to my extended deep sleep.

Sleep Music

Listening to sleep music or sleep noises certainly helped when going to sleep an there are many studies that bear this out. I found that I fell asleep quicker when I had music on in the background, and the most relaxing was actually thunder and rain, I now have 4 that I like to rotate through.

I will upload the black screen videos I found most soothing so you can try them out too.

Room Temperature

It took me about 2 months to get a temperature I liked, the a/c is now set to 23 degrees C, I use this to check it every night.

Dr. Matthew Walker states it is a scientific fact that your body and brain need to drop their core temp by around1°C (2 to 3°F) to initiate sleep, that’s why it is easier to fall asleep in a room that is too cold and not too hot.

I also take a warm shower before I go to sleep, this ensures that my blood rushes to the extremities and away from my core, when I get out of the shower there is then a massive dissipation of heat, out through the extremities and my core temperature plummets, so this adds to the cooling effect that everybody needs to sleep.

Food and Drink

Again, this took many months of adjusting the time I would eat and drink before going to bed. Also, what I would eat, was it a snack or a full meal, was a fat heavy meal or Carb heavy meal.

This was the most difficult; one night for example I would eat steak at 7.30 pm and have 25% of my sleep that night be deep; I would replicate it the next night only to find my deep sleep went down to 18%.

It wasn’t until I had perfected the dark sleep environment (curtains and mask), the relaxing music and the temperature that I realised that, what I ate and when I ate it made very little difference to my deep sleep levels at all.

Even alcohol, on the weekend I could drink 1 or 2 glasses of wine right up to 10 pm and still get over 25% deep sleep and no reduction in overall sleep either.

I think 1 or 2 glasses of wine, once or twice a week, over the period of a few hours isn’t excessive, that said, on special occasions like birthdays I have drunk more, and it has affected my overall and deep sleep.

My Sleep Statistics 2020

Remember my sleep on 29, 30 and 31 of October 2019 was good as far as overall time is concerned, 7:34, 7:49 and 7:10, but my deep sleep only accounted for 8% and 17% of the overall total.

Here on the 3rd, 4th and 5th of August 2020, even on two nights when I only had just over 5:30 minutes total sleep, my deep sleep was between 28% and 34%, up from 8% and 17%.

This marked change took me about 10 months to achieve.

My Sleep Statistics 2021

And just to show that it is still working, my sleep Stats from the 19th, 20th and 21st of January 2021 are (I am preparing and recording this video on the 21st of Jan).

My overall sleep was 7:36, 6:57 and 8:07

With deep sleep constituting 29%, 36% and 34% respectively  

Summary

So, if you too want to improve, not only your overall sleep, but far more importantly your deep, regenerating sleep, I would try these things in this order (show pictures and talk):

·     Sleep/fitness monitor/tracker

·     Temperature

·     Mask

·     Music

·     Curtains

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

DISCLAIMER: The Video Content has been made available for informational and educational purposes only. The video creator does not make any representation or warranties with respect to the accuracy, applicability, fitness, or completeness of the Video Content. The Video Content is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of your physician or other qualified health provider with any questions you may have regarding a medical condition. The video creator hereby disclaims any and all liability to any party for any direct, indirect, implied, punitive, special, incidental or other consequential damages arising directly or indirectly from any use of the Video Content, which is provided as is, and without warranties.

Signal to Noise by Scott Buckley https://soundcloud.com/scottbuckley

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/signal-to-noise

Music promoted by Audio Library https://youtu.be/of0Ei_T4isc

Fitness/Sleep Tracker: https://amzn.to/39T6kTR

Sleep Mask: https://amzn.to/2Y5ZFQK

Smart Light Bulbs: https://amzn.to/3sKhxPf

Thermometer & Humidity Gauge: https://amzn.to/3qH6rbZ

Blackout Curtains: https://amzn.to/2YsjiD3

Blackout Blind: https://amzn.to/2M53gMD

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

Renue by Science 10% Discount Code: MYNMN (https://renuebyscience.com/?rfsn=5692699.331801&coupon-code=MYNMN)

Renue by Science 15% Subscription Service Discount Code: MYNMNSUB

Youth & Earth 15% Discount Code: MYNMN (https://youthandearth.com?sca_ref=3766764.knrWl4O3cB)

ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

Please note that the links above are affiliate links, which means that if you use the code, I will receive a small commission, and if you do, may I say many thanks I advance for your support.

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

For adults in their 40s, sarcopenia is hardly noticeable—about 3% muscle mass is lost each decade. However, for those aged 65 years and older, muscle decline can become much more rapid, with an average loss of 1% muscle mass per year.

More importantly, sarcopenia is also marked by a decrease in strength, impaired gait, reduced physical activity and difficulty completing simple everyday tasks such walking, sitting down and standing up unaided.

The Future

The proportion of older adults aged 65+ is projected to more than double by the year 2060, this is driving research into the process of musculoskeletal decline.

Researchers at Colorado State University's Columbine Health Systems Center for Healthy Aging believe they have found an animal model that will help them better understand it and find ways to limit the symptoms.

The study, published in Frontiers in Physiology: Striated Muscle in October, is an example of using comparative medicine to understand human diseases and conditions.

Animal Models

Scientists often rely on animal models to mimic disease progression and study the prevention, diagnosis and treatment of medical conditions.

However, until now, no animal model has been able to fully capture all aspects of human musculoskeletal aging.

Karyn Hamilton

Karyn Hamilton, a professor in the Colorado State University's Department of Health and Exercise Science, and associate director at the Center for Healthy Aging said "Existing pre-clinical models either rely on unloading the muscle, meaning mimicking bedrest in an animal, or they must wait until the animals get really, really old, and even then they don't really get the same muscle aging phenotype as people do."

Dunkin Hartley Guinea Pig

In their work, Hamiliton's team found that the Dunkin Hartley guinea pig was a good candidate for a muscle aging model due to the animal's tendency to develop osteoarthritis at a young age.

The two conditions, osteoarthritis and sarcopenia, seem to be linked in humans; with advancing age, skeletal muscle dysfunction increases the risk for osteoarthritis, and osteoarthritis increases the risk for further muscle decline.

Hamilton’s team

Hamilton teamed up with Dr. Kelly Santangelo—an associate professor in the Department of Microbiology, Immunology, and Pathology at CSU, who has been studying primary osteoarthritis in Hartley guinea pigs for many years, also Associate Professor Raoul Reiser, also in Department of Health and Exercise Science, to understand how skeletal muscle changes as osteoarthritis progresses in guinea pigs.

The team hypothesized that those muscle changes may mimic human musculoskeletal aging.

Classic signs

Muscle composition also changes with age: fast-twitch fibers, which are larger and capable of exerting stronger forces, decrease in number, and slow-twitch fibers, which are smaller and less metabolically efficient, tend to increase.

Generally, when people "lose muscle mass," it's the large, fast-twitch fibers that are affected, also with decreasing muscle mass tends to come an increase in fat mass.

 As humans age, it may be that maintaining muscle function is more important than preventing loss of mass, so does this mean weight training and functional exercise as opposed to just bulking up with muscle? As an aside, I know many body builders who have plenty of muscle, but have a pondered gait and can’t scratch behind their neck.

Statement by Hamilton

"What we've learned over the decades is that age-related loss of muscle mass and age-related loss of muscle function don't always go hand in hand, you can do things to improve muscle mass, and you might not get an improvement in muscle function; you can do things to improve muscle function and may not get an increase in muscle mass."

Comparisons

The researchers compared muscle changes observed in Hartley guinea pigs with those in Strain 13 guinea pigs; Strain 13 guinea pigs tend to develop osteo-arthritis later in their lifespan and, therefore, might not exhibit the same classic signs of muscle aging.

They found some striking similarities to human muscle aging, such as a decrease in muscle density, likely due to an increase in fat mass.

While a decrease in muscle mass was not noted, researchers did find a shift toward smaller, slow-twitch muscle fibers, as is expected in human muscle with advancing age.

Hamilton

"If you look at the overall picture, we think that some of the key things that always happen with human muscle aging—that shift toward a less powerful, slower-twitch muscle phenotype—are quite clearly modeled in the Hartley guinea pigs. And we believe that if we started looking at even older guinea pigs, we might see more of the things that people think of as classic sarcopenia."

Future Directions

This study now provides a baseline that allows the team to take multiple directions in future research.

One direction will be to employ functional tests to study how muscle strength and gait, or mobility change with age in the Hartley guinea pigs, and how these changes mimic the deterioration of muscle function in aging humans.

 A priority will be to identify if Hartley guinea pigs can be a valuable translational model for identifying interventions that show promise for preventing or slowing the decline in overall musculoskeletal function in aging in humans.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/-lonely-soul​

Music promoted by Audio Library https://youtu.be/_ozYQF0iA6k

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

Renue by Science 10% Discount Code: MYNMN (https://renuebyscience.com/?rfsn=5692699.331801&coupon-code=MYNMN)

Renue by Science 15% Subscription Service Discount Code: MYNMNSUB

Youth & Earth 15% Discount Code: MYNMN (https://youthandearth.com?sca_ref=3766764.knrWl4O3cB)

ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Links: 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952478/

https://pubmed.ncbi.nlm.nih.gov/22508710/

https://www.acs.org/content/acs/en/pressroom/newsreleases/2015/march/vitamin-d-may-keep-low-grade-prostate-cancer-from-becoming-aggressive.html

https://journals.lww.com/oncology-times/fulltext/2015/04250/evidence_that_vitamin_d_can_slow_or_reverse.20.aspx

https://www.healthline.com/health/prostate-cancer/vitamin-d-and-prostate-cancer

https://www.cancer.northwestern.edu/news/2014/Murphy-Vitamin-D-Deficiency.html

https://www.medscape.com/viewarticle/842012

At the 249th National Meeting & Exposition of the American Chemical Society (ACS) it was announced that supplementing with Vitamin D supplements may slow or even reverse the progression of less aggressive, or low-grade, prostate tumors without the need for surgery or radiation.

Gleason Grading System

If a tumor is present in a prostate biopsy, the pathologist will grade its aggressiveness on a scale known as the Gleason Grading System.

Tumors attracting a score of 7 or above are considered aggressive and are prone to spreading; they will require either surgical removal of the prostate gland or radiation therapy.

However, tumors with a score of 6 or less are classed as less aggressive and in some cases may show no symptoms or cause no health problems for the duration of the man's life.

Active Surveillance

Bruce Hollis, Ph.D., who is at the Medical University of South Carolina, explained that in cases of a low-grade prostate cancer (a score of 6 or below), many urologists do not treat the disease, but instead initiate "active surveillance. 

The cure, which means surgery or radiation, is probably worse than the disease, so urologists will wait a year and then conduct another biopsy to see where the patient stands."

Elective Surgery

That said, knowing that they have even low-grade prostate cancer can cause patients and their family’s excessive anxiety, this may prompt some of the men to undergo elective surgery, despite the risk of complications such as infection, urinary incontinence and erectile dysfunction.

But a man must wait at least 60 days from the time of his biopsy before he can undergo surgery, this is that any inflammation caused by the biopsy can wane.

Vitamin D supplementation

Bruce Hollis wondered if giving these men vitamin D supplements during the 60-day waiting period would affect their prostate cancer.

Previous research had shown that when men with low-grade prostate cancer took vitamin D supplements for a year, 55% of them showed decreased Gleason scores, and in some of the cases the tumors disappeared completely.

Clinical Trial

In a new randomized, controlled clinical trial, Bruce Hollis’s team was assigned 37 men who were due to undergo elective surgery.

There were two groups, one that received 4,000 IU of vitamin D per day and a second placebo group that didn't receive vitamin D.

The men's prostate glands were removed and examined 60 days later.

Results

Hollis stated, “The new research suggests that vitamin D supplementation may improve low-grade prostate cancers by reducing inflammation, perhaps lessening the need for eventual surgery or radiation treatment.”

He went on to say…

·"We don't know yet whether vitamin D treats or prevents prostate cancer, at the minimum, what it may do is keep lower-grade prostate cancers from going ballistic."

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Signal to Noise by Scott Buckley https://soundcloud.com/scottbuckley

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/signal-to-noise

Music promoted by Audio Library https://youtu.be/of0Ei_T4isc

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/-lonely-soul​

Music promoted by Audio Library https://youtu.be/_ozYQF0iA6k

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

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My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Links:

https://singularityhub.com/2020/11/24/another-win-for-senolytics-fighting-aging-at-the-cellular-level-just-got-easier/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405395/

https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(19)30641-3/fulltext

https://www.nature.com/articles/s42255-020-00305-3

https://www.nature.com/articles/s41591-018-0092-9

https://www.nature.com/articles/s41591-018-0092-9

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911708/

https://www.nature.com/articles/s41587-020-00750-1

https://www.healtheuropa.eu/senolytic-drugs-can-this-antibiotic-treat-symptoms-of-ageing/100585/ 

Firstly, many thanks to Tao Plakrapong, for sending me a link to this article. 

This is a review of the article ‘Another Win for Senolytics: Fighting Aging at the Cellular Level Just Got Easier’ by Shelly Fan PhD and some other interesting information on senolytic trials.

Links in the description below to the article and the other links I used to make this presentation.

Hallmarks of Aging

In the article Shelly references the parable of the blind men and the elephant and likens it to Longevity. For those unfamiliar with the parable, in essence blind men who don’t know what an elephant is are asked to touch a different body part and then conceptualize what the animal is like, because of their limited experience and specific area of touch, each man has vastly different conclusion and they all believe that they’re description is correct. She says that aging, thanks to its complexity, is the bio-medical equivalent of the elephant.

In that researchers have focused on either one or another “hallmark” of aging, with some success, but not a number together.

Joining the Dots

For example, past research has shown us that energy production in our cells can become erratic and non-functional as we age. Cells programmed for apoptosis don’t die, but instead become zombie-like “senescent cells.

A "senescent cell" is a cell in our body whose telomeres have reached their "Hayflick Limit" i.e. its telomeres have become so short that the cell cannot divide anymore.

It is not a healthy cell anymore, it’s not exactly dead, but it is programmed for death, this cell death is called apoptosis, the Greek for self-eating, if it doesn’t die it starts to produce chemicals that increase inflammation and damage our DNA.

The key is to find out how other hallmarks of aging, such as lower NAD, inflammation and shortening telomeres interact with zombie cells, so we can see the whole picture.

The Study

A new study published in Nature Metabolism has starting to connect the dots. A study, in mice, linked-up three promising anti-aging pathways

·     Attacking senescent cells

·     Addressing inflammation

·     Tackling unreliable energy production in cells

All three elements point to one key factor that drives aging.

Healthy to Zombie Cell

Shelly likens individual cells to small cities; each with their own power plants and their ‘superstar’ molecular worker is Nicotinamide Adenine Dinucleotide (NAD). As we know NAD is a molecule that’s critical for helping mitochondria produce energy.

 As we age our cells start losing NAD and with reduction our mitochondria become less able to produce the energy we need, which in turn flips normal cellular function into dysfunction. At present this hypothesis has only been prove in mice.

One dysfunctional element is that cells programmed to die through apoptosis don’t die, are not consumed by the body as extra fuel, but instead hang-around as senescent or zombie cells.

These zombie cells then turn to the dark side, and start to leak an inflammatory cesspool of molecules called Senescence Associated Secretory Phenotypes (SASP) that “spread” harm and injury to adjacent healthy cells.

Senolytics

So, let’s look at senolytics. Senolytics are a group of compounds that destroy these “zombie” cells.

A study in old mice, the equivalent of a 90-year-old human, found that wiping out these zombie cells with two simple compounds increased their lifespan by nearly 40 percent.

Others using a genetic “kill switch” in mice found that destroying just half of their zombie cells helped the mice live 20% longer and also allowed them to have healthier kidneys, stronger hearts, more luscious fur, and higher than normal energy levels.

In the same way that some companies now sell NAD boosters like NMN and NR, pharmaceutical companies are investigating over a dozen potential senolytics in a race to bring one to market.

But is two better than one, could NAD boosters and senolytics be combined?

The Buck Institute

This new study, led by Dr. Judith Campisi and Dr. Eric Verdin at the Buck Institute for Research on Aging in California, asked if the lines between lower NAD and zombie cells could be connected.

 And asked could the connection be CD38?

If you have watched my video on raising NAD and lowering CD38, you will know that CD38 wreaks havoc by both boosting inflammation and destroying our beloved NAD.

CD38 Study

Using tissue from mice and humans, the team traced CD38 to a type of immune cell. These cells, called M1 macrophages (which literally means, ‘big eaters) are well known to increase inflammation in the body and cause DNA damage as we age.

When comparing fat tissue isolated from young and old mice, the team realized that M1 macrophages relentlessly pump out CD38 as the cells age, which in turn, breaks down the much-needed NAD.

Dr. Eric Verdin used the analogy of a sink and water source and said "Do NAD levels drop because of a faucet problem, i.e. our ability to make NAD, or is it a leaky sink problem, where aging cells break down NAD too fast. Our data suggests that, at least in some cases, the issue stems from the leaky sink.”

Which to me means we can make NAD okay; it’s just that it runs out before we can use it.

The Zombie Connection

This is what we know so far: aging triggers immune cells that pump out CD38, a chemical that destroys our NAD.  But, how can we stop CD38?

In an unexpected twist of events, the connection actually seems to be the zombie cells.

Remember, zombie cells leave a chemical evidence trace called Senescence Associated Secretory Phenotypes (SASP). They also change their ‘molecular structure’ so it’s possible to separate them from the healthy cells.

 In the fatty tissue gathered from aged mice, the team identified zombie cells and found that their “toxic waste” vastly increased the amount of CD38.

So, it is the Senescence Associated Secretory Phenotypes (SASP) that direct the immune cells to make more CD38.

Testing the Theory

So, if zombie cells are the directors, then getting rid of them should reduce CD38 and in turn, preserve our NAD; well that’s the theory.

To test it out, the team used genetically engineered mice; this allowed the scientists to identify the zombie cells and then selectively kill them.

The team injected the mice with a drug that damaged their DNA. This mimicked aging, in the sense that it increased zombie cells and CD38.

Result, killing the zombie cells lowered CD38 levels, which in turn preserved NAD.

Statement

Dr. Verdin said “We are very excited to link two phenomena which have been separately associated with aging and age-related disease.

For now, zombie cells seem to be a master-level culprit that drives inflammation, decreases NAD levels, and breaks the cell’s energy production.

This suggests that senolytics, which selectively kills off zombie cells, could as a secondary effect also increase NAD, something we didn’t know previously."

NAD Boosters

On supplementation Dr. Eric Verdin said, “Ultimately I think supplementation will be part of the equation, but filling the sink without dealing with the leak will be insufficient to address the problem.”

In other words, for NAD supplementation to work more efficiently, we may need to also use senolytics drugs to decrease zombie cells and CD38 levels; in essence plugging the leak in the sink, before we turn the NAD tap on

Where to get Senolytics?

In an article posted in the US National Library of Medicine, it states that Senolytics are a class of drugs that selectively clear senescent cells.

·     The first senolytic drugs

o  Dasatinib (approved in the USA and EU as a leukemia treatment)

o  Quercetin (a plant Flavonol available in supplement form)

o  Navitoclax (an experimental anti-cancer drug)

o  Fisetin (a dietary Flavonoid available in supplement form)

Early pilot trials of senolytics suggest they decrease senescent cells, reduce inflammation and alleviate frailty in humans

Clinical trials for diabetes, idiopathic pulmonary fibrosis, Alzheimer’s, COVID‐19, osteoarthritis, osteoporosis, eye diseases, bone marrow transplants and childhood cancer survivors are either underway or just beginning.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/-lonely-soul​

Music promoted by Audio Library https://youtu.be/_ozYQF0iA6k

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

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ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Links:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269139/#:~:text=Sarcopenia%20is%20a%20condition%20characterized,exclusively%20seen%20in%20older%20persons.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066461/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623318/

https://pubmed.ncbi.nlm.nih.gov/27370407/

https://pubmed.ncbi.nlm.nih.gov/27376823/

https://pubmed.ncbi.nlm.nih.gov/25365952/

https://pubmed.ncbi.nlm.nih.gov/26844538/

https://pubmed.ncbi.nlm.nih.gov/25826683/

https://pubmed.ncbi.nlm.nih.gov/26073423/

https://pubmed.ncbi.nlm.nih.gov/26980367/

https://pubmed.ncbi.nlm.nih.gov/26566405/

https://www.medicalnewstoday.com/articles/318501

https://www.thelancet.com/article/S0140-6736(19)31138-9/fulltext

https://www.health.harvard.edu/staying-healthy/preserve-your-muscle-mass

https://www.sciencedirect.com/science/article/pii/S1568163716302719

https://www.healthline.com/health/sarcopenia#prevention

https://www.healthline.com/nutrition/sarcopenia#TOC_TITLE_HDR_5

https://www.webmd.com/healthy-aging/guide/sarcopenia-with-aging

Sarcopenia is the loss of muscle mass specifically related to aging. It’s normal to lose some muscle mass as you age. However, sarcopenia describes severe muscle loss that strays from the norm. Sarcopenia affects your gait, your balance and your overall ability to perform daily tasks. For too long, researchers have believed that this deterioration in muscle was inevitable. But they’re now beginning to look into treatments that might prevent or slow down this process.

Symptoms

People with sarcopenia often experience weakness and lose stamina. This can affect their ability to carry out even the simplest of physical activities like walking or sitting. This reduction in activity then leads to further muscle mass loss and instability.

Causes

A common cause of sarcopenia is decreased physical activity throughout the day. However, although less frequent, some people with active lifestyles may also be diagnosed with sarcopenia, this suggests that there could be other reasons for the development of the disease.

 Researchers currently believe that other causes of sarcopenia could include:

·     a reduction in the nerve cells that send signals from your brain to tell your muscles to move

·     a lowering of your hormone levels

·     a decline in your body’s ability to convert protein to energy, remember this is one of the roles of NAD

·     not consuming enough daily calories and protein in order to maintain your muscle mass

Treatment - Exercise

The main treatment path for sarcopenia is exercise. Researchers have identified resistance training as the specific form of exercise that is most beneficial to people with sarcopenia. This training is designed to improve muscle strength and stamina and uses resistance bands or weights.

 Resistance training can also help balance your hormone levels.

It has been shown to improve our ability to turn protein into energy. These changes have in some cases been seen in only two weeks.

 The right intensity and frequency of exercise is vital, so you achieve the best results, without causing injury.

So, spending 45 minutes at the gym, but only training for 25 won’t cut it; trust me you’re only kidding yourself .

Hormone Replacement Therapy (HRT)

HRT can help to raise lean body mass, decrease abdominal fat, and prevent bone loss in women whose hormone levels have decreased during the menopause. However, the use of HRT is debatable, mainly because of an increased risk of some cancers and other severe health conditions.

Other Treatments

Some other treatments that are under investigation include:

·     growth hormone supplements

·     testosterone supplements

·     Vitamin D, remember to supplement with Vitamin K2 also to ensure the calcium goes to your bones and teeth and not into your soft tissues

·     medications for the treatment of metabolic syndromes

If these prove useful, they should be used in conjunction with resistance training, not in place of it.

Prevention

Lack of activity is the most common reason behind this condition. Just half an hour of moderate exercise each day, like jogging or even walking, will help keep your system working and fit. In order for exercise to be effective, proper nutrition is also important. 

Research has shown that consuming more protein may help older adults reduce their chance of sarcopenia

Supplements

Supplements have also proven useful in the prevention of sarcopenia. Some include:

·      Creatine, for increasing and maintaining muscle mass (I take)

·      Vitamin D, for maintaining bone and muscle tissues (I take)

·      Protein, to help preserve body mass (don’t take)

As before these should be used in addition to exercise and not instead of it.

Associated conditions

Sarcopenia has been linked to metabolic problems like:

·     type 2 diabetes

·     high blood pressure

·     Obesity

 These three conditions are a type of gateway, which put you at greater risk of the developing coronary heart disease, stroke and other conditions that affect the blood vessels.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/-lonely-soul​

Music promoted by Audio Library https://youtu.be/_ozYQF0iA6k

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

Renue by Science 10% Discount Code: MYNMN (https://renuebyscience.com/?rfsn=5692699.331801&coupon-code=MYNMN)

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Youth & Earth 15% Discount Code: MYNMN (https://youthandearth.com?sca_ref=3766764.knrWl4O3cB)

ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Source 1: https://www.youtube.com/watch?v=Py49yZFk0so

Source 2: https://www.youtube.com/watch?v=YqjWQ9CtI-U

Source 3: http://www.oprah.com/style/twins-and-aging-factors-that-affect-aging/all

What is Epigenetics?

Epigenetics is the science of how our DNA is controlled and how it’s affected by external factors; these external factors affect our health-span and lifespan.

Genetics is fixed from birth; we get our genes from our parents and they define us as being male or female, they control our eye colour, height etc.

This is what is measured by companies such as 23andMe.

We all share more than 99% of our DNA, however the remaining 1% varies from person to person; this is what makes us unique

 Epigenetics looks into the dynamic changes in our gene function overtime, changes that don’t involve a change in the underlying DNA sequence; these changes are influenced by external factors

Good to Know

So, how much can these external ‘epigenetic’ factors actually affect out lifespan and health-span?

 Someone who is at high risk of heart disease from a genetic predisposition, so there is a family history of heart disease, may have four times the chance of developing heart disease because it is in their genes; that is obviously bad, but compare that to someone who has a low risk of heart disease from a genetic pre-disposition, but is affected by external environmental and lifestyle factors, the risk to them is 5,000 fold, so 5,000 times more chance of getting heart disease because of the environment or the life choices they made.

So, there is a lot more risk from factors that are under our control, than from our genes,

 As David Sinclair says, ‘Only 20% of longevity is genetic, the rest is up to you, what will you do today?

You can Affect this

So, identical twins have the same DNA, but through lifestyle choices may well have vastly different health outcomes by the time they reach middle-age (Source 3), which twin smoked and didn’t use sunscreen?

Biological age testing allows us to read our epigenetic markers, markers that we can influence through changes to our lifestyle.

 Epigenetic markers change every time one of our cells divides, and even when our cells are not dividing, changes are still taking place to our epigenetic markers.

 We can affect, through lifestyle, the way that these markers change and through DNA methylation testing we can monitor our progress, either good or bad.

 It is not easy to do the right thing and there is no magic pill, targeted supplementation is only a small part of the answer.

Our Biological Age?

So, why should we check our biological age? Our Epigenetic age is a much better measure of our health, well-being, lifespan and health-span than our chronological age.

​Knowing our epigenetic or biological age allows ‘us’ - you and me - to move our healthcare needs from being reactive to being proactive;

We can become citizen scientists; we can take responsibility for our own longevity by measuring our health objectively at the molecular level

 And once we know our Epigenetic age, we can take measures to adjust it if necessary, I am guessing no matter what your biological age is, you would like to see it lower?

 The Slippery Slope

Epigenetics measures how well your body is aging, if your epigenetic age is higher than your biological age you are more at risk from age related diseases and all-cause mortality [Source 2]

David Sinclair often talks about gateway issues such as being pre-diabetic or having elevated blood pressure, these very rarely stand-alone forever, they usually open the door to type 2 diabetes and hypertension, which in turn lowers our bodies defenses and opens us up to even more serious diseases.

 A forty-year-old whose epigenetic age is fifty-five is considerably more at risk from age related diseases than say a forty year old whose epigenetic age is forty-two, this stands to reason.

DNA Methylation v Telomere Lengths

There are now many companies that measure telomere lengths to predict epigenetic age, this is Dr Tom Stubbs, the CEO of Chronomics a company that conducts DNA methylation tests.

There are some issues with the accuracy of telomere length testing, but I will let Dr Tom Stubbs the CEO of Chronomics explain the details.

So, there are upwards of 20 million markers available to build models that can more accurately predict age, so there is a lot more information available than the one marker that is used when estimating age using telomeres, this means that epigenetic testing is much more accurate for estimating someone’s biological age, so, in this case, bigger is better

​​Chronomics

We have already heard from the CEO of Chronomics, Dr. Tom Stubbs, but in outline Chronomics are a B2B company who sell their technology to other companies; DoNotAge.org is one of those companies, they also have a YouTube channel where you can gather more epigenetic age testing information.

You can see from this image that the number of biological markers used by Chronomics will give a significantly more accurate epigenetic age than that of their competition, this information was gathered by DoNotAge.org as they searched for the best B2B provider of an epigenetic age prediction service.

The closest competitors are Muhdo with 850,000 markers and Elysium with 100,000 markers.

The company at the bottom of the list, epi-age,com is the company that I used on my 56 birthday, before I knew what I know now, but mainly out of necessity, they were at the time the only company I could find that did international testing.

Out of the 28 million available markers that can be measured to predict age, they use 13, not 13,000, just 13, so I am not too sure how many months margin of error that allows for, Chronomics with 20 million markers claim an accuracy bracket of within 6 months.

 Obviously, this level of science and accuracy comes at a cost, prices should come down with time, but at present the most accurate test is still very expensive

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Signal to Noise by Scott Buckley https://soundcloud.com/scottbuckley

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Free Download/Stream: https://bit.ly/signal-to-noise

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I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

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ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Links:

https://pubmed.ncbi.nlm.nih.gov/31710095/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509089/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3951182/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088772/

A long-term clinical study from Denmark has shown that increased Nicotinamide Adenine Dinucleotide (NAD) levels and improved mitochondrial function results from NR supplementation in mice could not unfortunately be repeated in humans.

NR 12 Week Study in Humans

Studies in animals have shown that NAD boosters stimulate metabolic health by improving mitochondrial function in skeletal muscle, so far NR supplementation has only been shown to raise human NAD levels in blood.

Scientists from Denmark have published, in the Journal of Physiology, the results of a 12-week study where they tested the effects of NR on NAD levels and mitochondrial function in human skeletal muscle.

Metabolic Health

Our metabolic health depends on mitochondrial function, but as our NAD decreases with age so does our mitochondrial function.

Research has linked these declines with age-related diseases such as obesity, insulin resistance and type 2 diabetes, this in turn results in the defective regulation of our skeletal muscle metabolism.

Scientific Study

The team of scientists studied NR supplementation to see if it did in fact increase NAD levels and improve mitochondrial function in human skeletal muscle too.

The clinical trial consisted of 40 participants (men with obesity and insulin resistance)

 They were given 1000 mg NR or a placebo twice a day for 12 weeks, 2000 mg per day is a lot, the advised dose is around 300 mg a day.

Results

The results showed that NR supplements did not improve NAD levels in the participants’ skeletal muscle.

The study also concluded that NR supplementation did not affect the level of mitochondria in their skeletal muscle.

The team also measured the levels of citrate synthase, an enzyme that correlates to mitochondrial levels, and unfortunately also found no changes.

The team also checked to see if NR supplementation increased reactions that use oxygen to convert nutrients into cellular energy, they found that NR supplementation did not increase in the overall mitochondrial oxidative capacity in human skeletal muscle

Study Conclusion

The team stated, ‘our data does not support the hypothesis that dietary NR supplementation has significant impact on skeletal muscle mitochondria in obese and insulin-resistant men.’

They also stated, ‘Future studies on the effects of NR on human skeletal muscle may include both sexes and potentially provide comparisons between younger and older people.’

The NR community

The NR community has been very quiet about this study? Charles Brenner who is usually very vocal (in his tweets) when it comes to the lack of NMN human studies or rubbishes anything linked to David Sinclair such as resveratrol, seems to have lost the ability to Tweet.

What the study doesn’t tell us

This study is very specific, if you are a man with obesity and insulin resistance, NR will not improve mitochondrial function in your skeletal muscle, IMHO anyone commenting on this study and not talking about the specifics of this study is just guessing.

 This study was conducted specifically in men with obesity and insulin resistance, so does NR work on skeletal muscle if:

You are a woman with obesity and insulin resistance

If you are obese, but not insulin resistant or vice versa

If you are neither obese nor insulin resistant

 There still seems to be more questions than answers and let’s hope that in the future that these type of studies are also conducted with NMN

and that they test people with other age related diseases, not just obesity and insulin resistance.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

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Free Download/Stream: https://bit.ly/-lonely-soul​

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I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

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 DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

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ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

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My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

I finally managed to find a mutually agreeable time to interview the CEO of DoNotAge.org, Alan Graves, and discuss what the company’s plans are with regard to the future, as well as putting a few of the questions that subscribers have also asked me to ask on their behalf.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Signal to Noise by Scott Buckley https://soundcloud.com/scottbuckley

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/signal-to-noise

Music promoted by Audio Library https://youtu.be/of0Ei_T4isc

I hope you enjoy my content and find it interesting or informative, if so, please consider supporting the channel by using one of the affiliate links below:

  * Buy me a Kofi: https://ko-fi.com/mynmnexperiment

  * Patreon: https://bit.ly/3hhfjl5

  * SubscribeStar: https://bit.ly/3psYo23

Renue by Science 10% Discount Code: MYNMN (https://renuebyscience.com/?rfsn=5692699.331801&coupon-code=MYNMN)

Renue by Science 15% Subscription Service Discount Code: MYNMNSUB

Youth & Earth 15% Discount Code: MYNMN (https://youthandearth.com?sca_ref=3766764.knrWl4O3cB)

ProHealth 15% Discount Code: MYNMN (https://www.prohealth.com/)

DoNotAge 10% Discount Code: MYNMN (https://bit.ly/2VBDgNt)

My Current Anti-Aging Protocol:

·      1.5 grams of NMN (https://bit.ly/3c2Fxt8) 

·      1.5 grams of Trans-resveratrol (Tue, Thu & Sat) (https://bit.ly/3yxeqy2) 

·      500mg Metformin

·      1.5 grams of TMG (https://bit.ly/3oe1Ted) 

·      5,000 IU (International Units) of vitamin D3 (https://bit.ly/3P32hYH) 

·      120 mcg (micrograms) of vitamin K2 (Mk 7) (https://bit.ly/3PhkBgn) 

·      250mg Magnesium (L-Threonate) (https://bit.ly/3O4pZ5o) 

·      200mg high molecular weight hyaluronic acid (https://bit.ly/3P0Z4c2) 

·      2,400mg of Fisetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3P2rSB0) 

·      2,400mg of Quercetin, on the 1st, 2nd & 3rd of each month (https://bit.ly/3IzulAy)

·      Quercetin & Fisetin Periodic Dosing (https://bit.ly/3mw1IgN)

·      81mg of aspirin (https://bit.ly/3uFjtem) 

·      800mg SIRT6 Activator (https://bit.ly/3MyfA4J)

·      600mg DIM (https://bit.ly/3ZX9NsG)

·      800mg GlyNAC (https://shorturl.ac/7b75r)

Links:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143492/

https://pubmed.ncbi.nlm.nih.gov/9771862/

https://pubmed.ncbi.nlm.nih.gov/18525006/

https://pubmed.ncbi.nlm.nih.gov/30294301/

https://pubmed.ncbi.nlm.nih.gov/18400738/

https://pubmed.ncbi.nlm.nih.gov/25939935/

https://pubmed.ncbi.nlm.nih.gov/26035535/

https://pubmed.ncbi.nlm.nih.gov/21917864/

https://pubmed.ncbi.nlm.nih.gov/22043417/

https://pubmed.ncbi.nlm.nih.gov/26053339/

https://pubmed.ncbi.nlm.nih.gov/27071359/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798924/

https://pubmed.ncbi.nlm.nih.gov/24670344/

https://pubmed.ncbi.nlm.nih.gov/27478669/

https://pubmed.ncbi.nlm.nih.gov/17145139/

https://pubmed.ncbi.nlm.nih.gov/11706280/

https://pubmed.ncbi.nlm.nih.gov/14736022/

https://pubmed.ncbi.nlm.nih.gov/27719737/

https://www.nejm.org/doi/full/10.1056/NEJMc063341

https://journals.sagepub.com/doi/abs/10.1177/1533210110392952

https://www.health.harvard.edu/staying-healthy/taking-too-much-vitamin-d-can-cloud-its-benefits-and-create-health-risks

https://www.healthline.com/nutrition/vitamin-d-side-effects#2

https://www.insider.com/can-you-take-too-much-vitamin-d

https://www.webmd.com/vitamins-and-supplements/news/20190408/for-one-man-too-much-vitamin-d-was-disastrous

https://www.medicalnewstoday.com/articles/322602

https://www.mayoclinic.org/healthy-lifestyle/nutrition-and-healthy-eating/expert-answers/vitamin-d-toxicity/faq-20058108#:~:text=The%20main%20consequence%20of%20vitamin,the%20formation%20of%20calcium%20stones.

https://www.vitamindcouncil.org/for-health-professionals-position-statement-on-supplementation-blood-levels-and-sun-exposure/

As we know Vitamin D is extremely important for good health.

It plays several roles in keeping your body’s cells healthy and functioning the way they should.

Most people don’t get enough vitamin D, so supplements are common.

However, it’s also possible — although rare — for this vitamin to build up and reach toxic levels in your body.

Let’s look at 6 potential side effects of getting excessive amounts of this important vitamin.

Deficiency

Vitamin D is involved in calcium absorption, immune function, protecting our bones, muscles and heart health.

It occurs naturally in food and is also be produced by your body when your skin is exposed to sunlight.

Aside from fatty fish, there are few foods rich in vitamin D, so many foods have to fortified with Vitamin D.

Very few people get enough sun exposure to produce adequate levels of vitamin D.

Deficiency is very common, it's estimated that about 1 billion people worldwide don't get enough vitamin D. Get a blood test and only supplement with what you need.

Toxicity

However, taking extremely high doses of vitamin D3 for long periods MAY and I stress MAY lead to an excessive build-up in your body.

Vitamin D toxicity occurs when blood levels rise above 150 nanograms per milliliter (ng/ml).

Thankfully Vitamin D toxicity isn't common and occurs almost exclusively in people who take long-term, high-dose supplements without monitoring their blood levels. Get a blood test and only supplement with what you need.

Elevated blood levels

Achieving adequate levels of vitamin D in your blood may help boost your immunity and protect you from diseases like osteoporosis, cancer and in 2 recent studies against COVID-19 too; however, there isn't agreement on an optimal range for adequate levels.

Although a vitamin D level of 30 ng/ml is typically considered adequate, the Vitamin D Council recommends maintaining levels of 40–80 ng/ml and states that anything over 100 ng/ml may be harmful.

 While an increasing number of people are supplementing with vitamin D, it's rare to find someone with very high blood levels of this vitamin.

Medical studies

One recent study looked at data from more than 20,000 people over a 10-year period. It found that only 37 people had levels above 100 ng/ml. Only one person had true toxicity at 364 ng/ml.

 In one case study, a woman had a level of 476 ng/ml after taking a supplement that gave her 186,900 IU of vitamin D3 per day for two months. That is 47 times the generally recommended safe upper limit of 4,000 IU per day. This woman was admitted to the hospital after she experienced fatigue, forgetfulness, nausea, vomiting and slurred speech

 Although only extremely large doses can cause toxicity, even strong supporters of these supplements recommend an upper limit of 10,000 IU per day

Elevated blood calcium levels

Vitamin D helps your body absorb calcium from the food you eat.

However, if vitamin D intake is excessive, blood calcium may reach levels that can cause unpleasant and potentially dangerous symptoms.

Symptoms of high blood calcium levels (or hypercalcemia) include:

o  digestive distress, such as vomiting, nausea, and stomach pain

o  fatigue, dizziness and confusion

o  excessive thirst

o  and frequent urination

Case studies

The normal range of blood calcium is between 8.5 and 10.2 mg/dl.

 In one case study, an older man with dementia who received 50,000 IU of vitamin D daily for 6 months was repeatedly hospitalized with symptoms related to high calcium levels.

 In another, two men took improperly labeled vitamin D supplements, this lead to calcium levels of 13.2 and 15 mg/dl.

It took a year for their levels to normalize after they stopped taking the supplements.

Nausea, vomiting, and poor appetite

Many side effects of too much vitamin D are related to excessive calcium in the blood.

 These include nausea, vomiting and poor appetite, however, these symptoms don't occur in everyone with elevated calcium levels.

 One study followed 10 people who had developed excessive calcium levels after they had taken high-dose vitamin D to correct a deficiency. Four of them experienced nausea and vomiting and three of them had a loss of appetite; so 7 out of 10 DID encounter some type of side effect

 Similar responses to vitamin D mega-doses have been reported in other studies. One woman experienced nausea and weight loss after taking a supplement that was found to contain 78 times more vitamin D than the dose stated on the label.

Importantly, these symptoms occurred only in response to extremely high doses of vitamin D3, which led to calcium levels greater than 12 mg/dl.

 Get a blood test and only supplement when needed, you may not die from this, but you may be wasting good money on a supplement you don’t need.

Stomach pain, constipation, or diarrhea

Stomach pain, constipation and diarrhea are common digestive complaints that are often related to food intolerances or irritable bowel syndrome. However, they can also be a sign of elevated calcium levels caused by vitamin D toxicity.

 These symptoms may occur in those receiving high doses of vitamin D to correct a deficiency.

As with other symptoms, response appears to be individualized even when vitamin D blood levels are similarly elevated.

Case studies

 In one case study, a boy developed stomach pain and constipation after taking improperly labeled vitamin D supplements, whereas his brother experienced elevated blood levels without any other symptoms.

 In another case study, an 18-month-old child who was given 50,000 IU of vitamin D3 for 3 months experienced diarrhea, stomach pain and other symptoms.

Thankfully, these symptoms subsided after the supplementation was curtailed.

Bone Loss

Because vitamin D plays an important role in calcium absorption and bone metabolism, getting enough is crucial for maintaining strong and healthy bones.

However, too much vitamin D can be detrimental to bone health.

 Although many symptoms of excessive vitamin D are attributed to high blood calcium levels, some researchers suggest that mega-doses may also lead to low levels of vitamin K2 in the blood.

This is important because one of vitamin K2's most important functions is to keep calcium in our bones and teeth and out of our sift tissues.

 To protect against bone loss, avoid taking excessive vitamin D supplements and take a vitamin K2 supplement too.

 You can also eat foods rich in vitamin K2, such as grass-fed dairy and meat, vegans may need to supplement.

Kidney Injury

Excessive vitamin D intake can also result in kidney injury.

In one case study, a man was hospitalized for kidney failure, elevated blood calcium levels and other symptoms that occurred after he received vitamin D injections prescribed by his doctor.

Indeed, most studies have reported moderate-to-severe kidney injury in people who develop vitamin D toxicity.

 In one study of 62 people who had received an excessively high-dose of vitamin D through injections; all 62 experienced kidney failure; whether they had healthy kidneys or an existing kidney disease.

Even if you follow a healthy diet, you may require supplements to achieve optimal Vitamin D levels. However, it’s also possible to have too much of a good thing

Make sure to avoid excessive doses of vitamin D. Generally, 4,000 IU or less per day is considered safe, but only as long as your blood values are being monitored.

In addition, make sure you purchase supplements from reputable manufacturers to reduce the risk of accidental overdose due to inaccurate labeling.

 If you've been taking vitamin D supplements and are experiencing any of the symptoms I have mentioned, please consult a healthcare professional as soon as possible.

Get a blood test and only supplement with what you need.

FAIR-USE COPYRIGHT DISCLAIMER

Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, commenting, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.

Lonely Soul by Rexlambo https://soundcloud.com/rexlambo​

Creative Commons - Attribution 3.0 Unported - CC BY 3.0

Free Download/Stream: https://bit.ly/-lonely-soul​

Music promoted by Audio Library https://youtu.be/_ozYQF0iA6k