TWiV explains that a recent report suggesting that the SARS-CoV-2 spike protein inhibits V(D)J recombination in vitro would not impact immunity after infection or vaccination, and describes the isolation of remdesivir resistant mutants in cells in culture, and the emergence of amino acid changes in the spike protein identical to those in variants of concern, in the absence of immune selection. Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Click arrow to play Download TWiV 830 (67 MB .mp3, 112 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Spike protein inhibition of V(D)J recombination in vitro (Viruses) Evolutionary predictability of SARS-CoV-2 (PLoS Path) Letters read on TWiV 830 Timestamps by Jolene. Thanks! Weekly Picks Kathy – When do we get back to normal? Maybe now. Rich – The Covid-19 Pandemic Alan – Yaupon Brothers American tea Vincent – Cracking the genetic code (and Niremberg’s account) Listener Picks Justin – Partisan vaccination Lisa – The Red Flag Intro music is by Ronald Jenkees Send your virology questions and comments to [email protected]