The CLSI M100 gets updated every year—but what do those changes actually mean for the microbiology lab?

In this episode of Let's Talk Micro, Luis is joined by Dr. April Bobenchik to break down the latest updates in the CLSI M100 2026 (36th edition) and how they translate into real-world lab practice.

They walk through important breakpoint changes, including aztreonam–avibactam and updates for Acinetobacter, along with new additions like trimethoprim-sulfamethoxazole breakpoints for beta-hemolytic streptococci. Another notable update this year is the updated anaerobic antibiogram and how it can be used to help guide decisions in the lab. The conversation also touches on antimicrobial tiers, why some breakpoints are temporarily removed, and practical ways labs can approach implementing new updates.

If you work in clinical microbiology, this episode will help you stay current and confident with the latest M100 changes.

🔗 Resources

Access CLSI M100 for free: https://em100.edaptivedocs.net/Login.aspx

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