Real Science Exchange

Perspective and Commentary: Variation in nutrient composition of feeds and diets and how it can affect formulation of dairy cow diets with St-Pierre & Weiss


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Dr. Weiss and Dr. St-Pierre co-authored this episode’s journal club paper in Applied Animal Science (ARPAS Journal). Bill and Normand share a career-long interest in how feedstuffs and diet variation impact cows. (6:31)

Bill and Normand discuss sources of variation, which they divide into true variation and observer variation. True variation means the feed has changed: a different field, change during storage, etc. Observer variation includes sampling variation and analytical variation. Some feeds may exhibit a lot of true variation and others may exhibit a lot of observer variation. And some feeds are high in both types of variation. Highly variable feeds should be sampled more frequently. Some feeds are so consistent that using book values makes more sense than sending in samples for analysis. Bill and Normand go on to give some examples and share sampling and analysis tips for different types of feedstuffs. (12:41)

Bill would often be asked if users should continue to average new samples with older ones or just use the new numbers from the most recent sample. He and Normand debate the pros and cons of the two approaches as well as discuss the use of a weighted average where recent samples would be weighted to contribute more. (26:02)

Next, our guests discuss how multiple sources of a nutrient reduce the TMR variation for that specific nutrient. For example, alfalfa NDF is more variable than corn silage NDF on average. Yet if you use a blend of these two ingredients, you end up with less variation in NDF than if you used all corn silage. Normand details the mathematical concepts behind this relationship. Both Bill and Normand emphasize that diets must be made correctly for the best results. (32:26)

How do feedstuffs and diet variations impact cows? Both guests describe different experiments with variable protein and NDF concentrations in diets. Some were structured, like alternating 11% CP one day and 19% CP the next for three weeks. Some were random, like randomly alternating the NDF over a range of 20-29% with much higher variation than we’d ever see on-farm. The common thread for all these experiments is that the diet variations had almost no impact on the milk production of the cows. (38:04)

Clay asks how variation in dry matter might affect cows. Bill describes an experiment where the dry matter of silage was decreased by 10 units by adding water. Cows were fed the wet silage for three days, twice during a three-week study. To ensure feed was never limited, more as-fed feed was added when the wet silage was fed. It took a day for cows on the wet silage treatment to have the same dry matter intake (DMI) as the control cows and milk production dropped when DMI was lower. However, when switching abruptly back to the dry silage diet, DMI increased the day following the wet silage and stayed high for two days, so the cows made up for the lost milk production. Bill and Normand underline that it is critical for the cows not to run out of feed and described experiments where feed was more limiting, yielding less desirable outcomes. (46:17)

In the last part of the paper, our guests outlined seven research questions that they feel need to be answered. Normand shares that his number one question is how long will cows take to respond to a change in the major nutrients? He feels that we spend an inordinate amount of money on feedstuffs analysis, and there are some feeds we should analyze more and some feeds we should quit analyzing. Bill’s primary research question revolves around controlled variation. What happens if you change the ratio of corn silage and alfalfa once a week? Will that stimulate intake? Data from humans, pets, and zoo animals indicate that diet variation has a positive impact and Bill finds this area of research intriguing. (50:43)

In closing, Clay encourages listeners to read this paper (link below) and emphasizes the take-home messages regarding sampling and research questions. Normand advises that if you are sampling feed, take a minimum of two samples, and try as much as you can to separate observer variation from true variation. He also reminds listeners to concentrate on a few critical nutrients with more repeatability for analyses. Bill encourages nutritionists to sit down and think when they get new data - before they go to their computer to make a diet change. If something changed, why did it change, and is it real? Take time to think it through. (1:01:38)

You can find this episode’s journal club paper from Applied Animal Science here: https://www.appliedanimalscience.org/article/S2590-2865(24)00093-4/fulltext

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Real Science ExchangeBy Balchem Animal Nutrition & Health

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