Question: What cofactors are needed to synthesize and recycle BH4? Short Answer: Zinc, magnesium, potassium, and niacin are the cofactors needed for the synthesis and recycling of BH4. Folate and methylation are not involved, though high-dose folate or folic acid could hypothetically hurt BH4 recycling since both are recycled by dihydrofolate reductase (DHFR). This is a clip from a live Q&A session open to CMJ Masterpass members. In addition to this episode, you can access two other free samples using this link:

https://chrismasterjohnphd.substack.com/p/questions-on-vitamin-d-sulfate-synthesis

In that batch of free episodes you will also find the answers to these questions:

• Is It Important to Get Vitamin D Sulfate Specifically From the Sun?

• How Much Iron Can We Absorb At Once?

If you want to become a Masterpass member so you can participate in the next live Q&A, or so you can have access to the complete recording and transcript of each Q&A session, you can save 10% off the subscription price for as long as you remain a member by using this link to sign up:

https://chrismasterjohnphd.substack.com/qanda

Learn more about the Masterpass here:

https://chrismasterjohnphd.substack.com/about

This snippet is from the February 15, 2023 AMA. The full recording and transcript is reserved for Masterpass members. Here is a preview of what's included:

• Is It Important to Get Vitamin D Sulfate Specifically From the Sun?

• How Much Iron Can We Absorb At Once?

• What nutrients are important for long-term PPI use?

• For how long does transferrin saturation respond to recent iron-rich food?

• Muscle spasms: creatine, creatinine, sodium, and potassium.

• Hematologists ignore iron saturation.

• How to detox arsenic?

• Could folic acid supplements impair BH4 recycling?

• How to increase butyrate?

• More on hematologists and transferrin saturation.

Here's a link to the full AMA: https://chrismasterjohnphd.substack.com/p/recording-and-transcript-of-the-february

Access the show notes, transcript, and comments here.

Chris Masterjohn, PhD, is the Founder and Scientific Director of the mitochondria test Mitome.