In this episode, we discuss the anatomy and physiology of the stomach and its unique epithelial structure. We dive into the pathophysiological implications of conditions such as Helicobacter pylori infection and hypochlorhydria, examining how they disrupt gastric function and microbial balance. Finally, we connect these concepts to the small intestine, discussing possible downstream effects of altered gastric acid secretion, focusing on microbial colonization and contributions to broader gastrointestinal health challenges, such as small intestinal bacterial overgrowth (SIBO).

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Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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This episode explores three top topics from episodes in 2024: the mucus layer's protective role in maintaining gut barrier integrity, the bidirectional relationship between the microbiota-gut-brain axis and the hypothalamic-pituitary-adrenal (HPA) axis, and the importance of methylation and the MTHFR gene. During the first topic of discussion, we detail ways to support the intestinal mucus layer; as we approach the third topic of discussion, we highlight the impact of MTHFR enzyme activity on methylation, emphasizing its importance for many cellular processes.

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Thank you to our episode sponsor:

1. Check out ⁠Ulyana Organics'⁠ ⁠Tallow Wild Yam Cream⁠ and ⁠Healing Facial Oil⁠, and use code ⁠CHLOE10⁠ 10% off your order.

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Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we go through the interplay between the oral microbiome, the intestinal microbiome, and brain health, focusing on the mechanisms through which dysbiosis in these ecosystems influences neuroinflammation and central nervous system function. We explore the molecular pathways by which bacterial metabolites and inflammatory mediators from the oral and intestinal microbiota can disrupt the blood-brain barrier, activate glial cells, and impair neuronal processes. We also go through symptoms associated with microbial imbalance as well as oral health practices.

Thank you to our episode sponsor:

1. Check out Ulyana Organics' Tallow Wild Yam Cream and Healing Facial Oil, and use code CHLOE10 10% off your order.

Thanks for tuning in!

Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we dive into the interplay between the gut microbiota (their postbiotics) and the immune system. We will examine how gut microbiome-derived metabolites, such as short-chain fatty acids (SCFAs) and polyphenol metabolites, influence immune function by modulating key cellular and molecular pathways within the intestinal mucosa. Additionally, we discuss strategies for supporting butyrate production and optimizing microbiome health to foster a balanced and resilient gut-immune axis.

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11. Specific Polyphenols

"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we explore the intricate role of the vagus nerve as a central regulator within the microbiota-gut-brain (MGB) axis, examining its neuroanatomical structure, signaling mechanisms, and interactions with microbial metabolites and immune pathways. We discuss how vagal afferent fibers relay sensory input from the gut to the brain, including signals mediated by short-chain fatty acids (SCFAs) and gut-derived hormones, and how efferent fibers modulate gut motility, intestinal barrier integrity, and inflammation through the cholinergic anti-inflammatory pathway. Finally, we explore vagal dysfunction as well as associated conditions and symptoms, and we touch on just a few potential root causes.

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"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we dive into the interplay between the hypothalamic-pituitary-adrenal (HPA) axis and the microbiota-gut-brain (MGB) axis, highlighting their bidirectional communication through endocrine, immune, and neural pathways. We’ll explore how gut-derived metabolites like short-chain fatty acids (SCFAs) and endotoxins like LPS influence HPA axis activity; conversely, we explore how dysregulated cortisol can impact gut barrier function, immune signaling, and more. We also discuss testing including stool analysis and DUTCH tests.

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"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we examine the bidirectional relationship between intestinal health and immune aging, emphasizing how gut barrier dysfunction and microbial imbalances accelerate systemic inflammation and diminished immune function. Furthermore, we explore the effects of aging on intestinal barrier function, focusing on the decline in tight junction integrity, reduced mucus production, and impaired epithelial renewal, all of which contribute to increased gut permeability and chronic inflammation. Thus, restoring microbial diversity, supporting mucosal defenses, and addressing gut permeability are essential strategies to break this cycle and promote healthy aging.

Topics:

1. Introduction: Immune Aging

- Immune aging: a decline in immune function, increasing vulnerability to infections and chronic diseases.

- The intestinal microbiome influences and is shaped by immune aging.

- Microbial metabolites, epithelial integrity, and immune signaling are critical.

2. Anatomical Context of the Gut and Immune Cells

- Gut layers: lumen, mucus, epithelium, lamina propria, and more.

- The mucus layer protects the epithelium; function can become impaired with age.

- Tight junctions prevent pathogen entry, while the lamina propria houses immune cells.

3. Mucus Layer and Goblet Cells: Role and Aging Effects

- Goblet cells secrete mucins that form the protective mucus layer.

- Aging can reduce mucin production, weakening the mucus barrier.

- A thin mucus layer increases epithelial exposure and inflammation risk.

4. Microbial Interactions with the Mucus Layer

- Commensal bacteria support mucus turnover by controlled mucin degradation.

- Dysbiosis can disrupt this process, thinning the mucus layer.

- Reduced mucus protection increases susceptibility to pathogens.

5. Effects of Immune Aging on the Intestinal Barrier

- Aging effects on T cells, B cells, and inflammatory cytokine secretion.

- Cytokines disrupt tight junctions and increase permeability.

- Microbial products like LPS (endotoxin) cross the barrier, fueling chronic inflammation.

6. Dysbiosis and Its Role in Immune Aging

- Dysbiosis reduces SCFA production and Treg activity.

- Dysbiosis promotes chronic inflammation and accelerates immune aging.

7. Microbial Translocation and Systemic Effects

- A weakened barrier allows microbial products to reach immune cells.

- LPS triggers inflammatory signaling, amplifying systemic inflammation.

- Chronic inflammation perpetuates gut dysfunction and immune aging.

8. Conclusion

- Immune aging and intestinal health are interconnected in a feedback loop.

- Aging weakens the gut barrier, while dysbiosis amplifies inflammation.

- Restoring microbial balance and gut integrity is vital for healthy aging.

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"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In today’s episode, we dive into the interplay between the gut and immune system, focusing specifically on the critical role of immunoglobulins in mucosal immunity. Immunoglobulins, specialized glycoproteins synthesized by B cells, serve as the frontline defenders within the gastrointestinal tract, where they neutralize pathogens and maintain immune homeostasis. We also explore the mechanisms by which these antibodies fortify the intestinal barrier and aid in preventing dysbiosis. We finish by going through tools for supporting immunoglobulins.

Topics:

1. Introduction

- Overview of the gut-immune axis and focus on immunoglobulins.

- How low levels of immunoglobulins can contribute to dysbiosis.

2. What Are Immunoglobulins?

- Immunoglobulins (Igs) are glycoproteins produced by B cells.

- They play a critical role in recognizing and neutralizing pathogens.

- Antigen-binding sites for specific targeting.

- Essential for immune homeostasis and pathogen elimination.

3. Immunoglobulins’ Role in the Gut

- Protect mucosal surfaces exposed to antigens.

- B cells differentiate into plasma cells, and secrete immunoglobulins into the gut lumen.

4. Layers of the Intestinal Lining

- Intestinal lumen is covered by a protective mucus layer.

- Mucosa consists of epithelial cells, including mucus-secreting goblet cells.

- Lamina propria beneath the epithelium is rich in immune cells.

- Submucosa beneath mucosa.

5. The Function of the Mucus Layer

- Acts as a physical barrier against pathogens.

- Functions as a biochemical barrier by housing antimicrobial peptides and immunoglobulins.

- Primarily composed of mucins secreted by goblet cells.

- Goblet cells release mucins in response to stimuli, forming gel-like mucus.

6. Introduction to Secretory Immunoglobulin A (sIgA)

- sIgA is the most abundant immunoglobulin in mucosal secretions.

- Forms a protective barrier in the mucus layer.

- Plays a critical role in immune exclusion by trapping and neutralizing pathogens.

- Helps maintain non-inflammatory defense at mucosal surfaces.

7. Production of Secretory IgA

- Plasma cells in the lamina propria secrete IgA.

- IgA binds to receptors on epithelial cells and is transported across to the gut lumen.

- sIgA to neutralize pathogens effectively within the mucus layer.

8. Functions of Secretory IgA in the Gut

- Binds to antigens like bacterial walls, viruses, and toxins.

- Traps antigens in the mucus to prevent adherence to epithelial cells.

- Provides immune exclusion, reducing pathogen access to the gut lining.

- Maintains mucosal barrier integrity to prevent inflammation.

9. Impact of Low sIgA Levels

- Contributes to dysbiosis by allowing pathogenic bacteria to proliferate.

- Intestinal hyperpermeability.

- Increased risk of intestinal inflammation and infections.

10. Comparison with Other Immunoglobulins: IgG and IgM

- IgG is most abundant in blood.

- IgM is produced early in immune responses.

- IgM can also be secreted into the gut lumen in IgA deficiency.

11. Immunoglobulin Supplements

- IgG supplements.

- Colostrum: IgG to help neutralize pathogens.

- Supports gut barrier integrity.

12. Supporting Immunoglobulin Levels

- Colostrum

- Probiotics, prebiotics

- Vitamin A

- Zinc

13. Conclusion

- Immunoglobulins, intestinal health, and immune balance.

Thanks for tuning in!

"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In today’s episode, we dive into the critical role of phospholipids in maintaining brain health, examining how these molecules contribute to neuronal communication, synaptic plasticity, and cognitive resilience. We'll explore the biochemical structure and function of key phospholipids like phosphatidylserine and phosphatidylcholine within neuronal membranes, where they play indispensable roles. We discuss associations and impacts of environmental toxins, aging, and neurodegenerative conditions. Finally, we discuss dietary tools to support phospholipid levels and promote cognitive health.

Topics:

1. Introduction to Phospholipids and Cognitive Health

2. Brain Cell Structure and Composition

- Neurons, glial cells.

3. Detailed Anatomy of Neurons

- Soma contains organelles, dendrites receive signals, axon sends impulses.

- Myelin sheath insulates axon, speeding signal transmission.

- Myelin is rich in phospholipids.

4. Roles of Glial Cells in Brain Health

- Astrocytes, oligodendrocytes, and microglia support neurons.

- Glial cells regulate the brain’s environment, form myelin, and provide immune defense.

- Phospholipid-rich membranes are essential for glial function.

5. Biochemistry: Phospholipids

- Phospholipids have a glycerol backbone, fatty acid tails, and a phosphate group.

- Hydrophilic and hydrophobic parts form bilayers.

- Key phospholipids: PC, PE, PS, PI

6. Phospholipid Bilayer’s Role in Neuronal Communication

- Ion channels, receptors, and transporters in the bilayer enable cell functions.

- Ion channels allow ions to flow, creating signals for neuron communication.

- Receptors detect neurotransmitters, initiating responses.

7. Neuronal Activation and Electrochemical Gradients

- Resting neurons have ion concentration differences inside and outside the cell.

- Ion channel activity during activation creates an action potential.

- The phospholipid membrane enables controlled ion flow for signal transmission.

8. Neurotransmitter Release

- Action potential at axon terminal triggers calcium entry.

- Calcium causes vesicles to release neurotransmitters.

- Released neurotransmitters bind to receptors, continuing the signal.

9. Diversity of Phospholipids in Neuronal Membranes

- Different phospholipids (PC, PE, PS, PI) are essential for membrane integrity.

10. Summary: Phospholipids in Brain Function and Cognitive Health

- Phospholipids support neuronal communication, synaptic plasticity, and cognitive resilience.

- Synaptic plasticity - essential for learning and memory.

11. Phospholipid Disruption and Cognitive Decline

- Oxidative stress, aging, and inflammation disrupt phospholipid composition.

- Lipid peroxidation damages membranes, affecting neuron signaling.

- Phospholipid damage contributes to cognitive decline.

12. Importance of Phospholipids in Aging and Brain Health

- Lipid levels decrease with age, impacting brain function.

13. Environmental Toxins and the Brain

- Heavy metals like mercury cause oxidative damage to phospholipids.

- Damaged phospholipids and impaired neuron function.

14. Consequences of Suboptimal Phospholipids

- Cognitive symptoms.

- Low levels seen in neurodegenerative conditions.

15. Tools for Supporting Phospholipid Levels

- Foods with PS and PC, such as fatty fish and eggs.

- Phospholipid supplements.

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"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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In this episode, we explore serotonin synthesis within enterochromaffin (EC) cells in the gut, detailing how tryptophan is converted into serotonin through enzymatic processes. We examine the role of gut microbiota-derived metabolites, such as short-chain fatty acids (SCFAs), in modulating the synthesis of serotonin including impacting tryptophan hydroxylase activity. We explore serotonin's interactions with receptors on enteric neurons and vagal afferent fibers, analyzing how these signaling pathways influence gut motility. Finally, we uncover conditions and symptoms associated with low serotonin production and the importance of the intestinal microbiome.

Topics:

1. Introduction to Gut-Produced Serotonin

- Serotonin production within the gut.

- Serotonin’s role beyond mood.

- Synthesis, causes of low serotonin, related GI symptoms.

2. Gut Lining Structure and Cell Types

- Layers of the gut lining, focusing on the mucosa.

- Description of epithelial cells, including enterocytes, goblet cells, Paneth cells, and enteroendocrine cells.

- Role of enterochromaffin (EC) cells in serotonin production.

3. Serotonin Synthesis in EC Cells

- Location and function of EC cells.

- How EC cells synthesize serotonin from tryptophan.

4. Biochemical Pathway of Serotonin Production

- Step-by-step process: conversion of tryptophan to serotonin.

- Enzymes involved, including TPH1 and AADC.

- Importance of tryptophan availability in serotonin synthesis.

5. Storage and Release of Serotonin in EC Cells

- Role of VMAT1 in serotonin storage within vesicles.

- Controlled release.

6. Triggers for Serotonin Release

- Physical triggers: mechanical stretch, pressure from food intake.

- Chemical triggers: microbial metabolites, bile acids.

- Receptors involved (GPCRs, TGR5) and signaling pathways.

7. Release of Serotonin into Intestinal Lining Layers

- Serotonin exocytosis and interaction with nearby cells.

- Release of serotonin on both luminal and basolateral sides of EC cells.

- How luminal and basolateral release affects gut motility and barrier function.

8. Serotonin’s Role in Gut Motility

- Interaction with 5-HT3 and 5-HT4 receptors on enterocytes and enteric neurons.

- Activation of the enteric nervous system (ENS) in the submucosal and myenteric plexuses.

- Coordination with pacemaker cells for peristaltic movement.

9. Immune Function and Serotonin in the Gut

- Effect on immune cells.

10. Gut-Brain Communication via Serotonin and the Vagus Nerve

- Activation of vagal afferent fibers by serotonin.

11. Contributing Factors to Low Serotonin Production

- Impact of dysbiosis and reduced SCFA production.

- SIBO specifically.

- Intestinal inflammation in general.

- Imbalanced microbiota and inflammation can disrupt EC cell function.

12. Manifestations of Low Serotonin in the Gut

- Effects on motility: constipation, dysmotility...

- Common GI symptoms, including bloating, discomfort, and fullness.

- Association with conditions like IBS.

13. Supporting Serotonin Production in the Gut

- Painting a full picture and identifying root causes.

- Strategies to foster a healthy gut microbiome.

- Role of sunlight and tryptophan-rich foods in serotonin production.

- Stool testing for microbiome imbalances.

Thanks for tuning in!

"⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠"

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Visit ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠synthesisofwellness.com⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠